Department of Infectious Disease, Nanjing Drum Tower Hospital Clinical College of Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210008, P.R. China.
Mol Med Rep. 2013 Aug;8(2):669-75. doi: 10.3892/mmr.2013.1542. Epub 2013 Jun 25.
Polysaccharopeptide (PSP) is well known for its immunoregulatory effects. In the present study, the effect of PSP on white blood cell (WBC) count, T lymphocyte subsets, B lymphocytes, Th1/Th2 balance and negative immune regulators was investigated using an immunosuppressed mouse model. The results demonstrated that the WBC count and the absolute number of CD3+CD4+ T cells, CD3+CD8+ T cells and CD3‑CD19+ B cells in the peripheral blood were increased in PSP‑treated groups as compared with the cyclophosphamide (Cy) group. In addition, PSP reduced interleukin (IL)‑4 and GATA binding protein 3 (GATA‑3) mRNA relative expression levels and elevated the ratios of IL‑2/IL‑4 and the transcription factors, T‑box‑containing protein/GATA‑3. The relative mRNA expression levels of the forkhead/winged‑helix transcription factor box protein 3 (Foxp3), programmed death‑1 (PD‑1) and IL‑10 were also downregulated by PSP. These observations indicate that the immunoregulatory effects of PSP are associated with restoration of WBC number, improving the absolute number of T lymphocyte subsets and B lymphocytes, inducing the Th1/Th2 response and downregulating the negative immune regulators, Foxp3, PD‑1 and IL‑10.
多糖肽（PSP）以其免疫调节作用而闻名。在本研究中，采用免疫抑制小鼠模型研究了 PSP 对白细胞（WBC）计数、T 淋巴细胞亚群、B 淋巴细胞、Th1/Th2 平衡和负性免疫调节剂的影响。结果表明，与环磷酰胺（Cy）组相比，PSP 治疗组的外周血 WBC 计数和 CD3+CD4+T 细胞、CD3+CD8+T 细胞和 CD3-CD19+B 细胞的绝对数均增加。此外，PSP 降低了白细胞介素（IL）-4 和 GATA 结合蛋白 3（GATA-3）mRNA 的相对表达水平，并升高了 IL-2/IL-4 比值和转录因子 T 盒结合蛋白/GATA-3。叉头/翼状螺旋转录因子盒蛋白 3（Foxp3）、程序性死亡-1（PD-1）和 IL-10 的相对 mRNA 表达水平也被 PSP 下调。这些观察结果表明，PSP 的免疫调节作用与恢复白细胞计数、提高 T 淋巴细胞亚群和 B 淋巴细胞的绝对数、诱导 Th1/Th2 反应以及下调负性免疫调节剂 Foxp3、PD-1 和 IL-10 有关。
