Guangdong Provincial Key Laboratory of Animal Nutrition Control, College of Animal Sciences, South China Agricultural University, Guangzhou 510642, China.
Guangdong Province Key Laboratory of Waterfowl Healthy Breeding, Guangzhou 510225, China.
Molecules. 2018 Apr 17;23(4):932. doi: 10.3390/molecules23040932.
Polysaccharide of Koidz (PAMK) has been well recognized as an immune enhancer that can promote lymphocyte proliferation and activate immune cells. The purpose of this study was to evaluate the effects of PAMK on humoral and cellular immune functions in immunosuppressed geese. Geese of the Control group were provided with normal feed, the PAMK group was provided with 400 mg·(kg body weight) PAMK, the cyclophosphamide (CTX) group was injected with 40 mg·(kg body weight) cyclophosphamide, while the CTX+PAMK group received the combination of PAMK and CTX. Spleen development and percentages of leukocytes in peripheral blood were examined. Principal component analysis was conducted to analyze correlations among humoral and cellular immune indicators. The results showed that PAMK alleviated the damage to the spleen, the decrease in T- and B-cell proliferation, the imbalance of leukocytes, and the disturbances of humoral and cellular immunity caused by CTX. Principal component analysis revealed that the relevance of humoral-immunity-related indicators was greater, and the CTX+PAMK group manifested the largest difference from the CTX group but was close to the Control group. In conclusion, PAMK alleviates the immunosuppression caused by CTX in geese, and the protective effect on humoral immunity is more obvious and stable.
昆布多糖（PAMK）已被公认为一种免疫增强剂，可促进淋巴细胞增殖并激活免疫细胞。本研究旨在评估 PAMK 对免疫抑制鹅体液和细胞免疫功能的影响。对照组鹅给予常规饲料，PAMK 组给予 400mg·（kg 体重）PAMK，环磷酰胺（CTX）组给予 40mg·（kg 体重）CTX，CTX+PAMK 组给予 PAMK 和 CTX 的组合。检查脾脏发育和外周血白细胞百分比。进行主成分分析以分析体液和细胞免疫指标之间的相关性。结果表明，PAMK 减轻了 CTX 引起的脾脏损伤、T 细胞和 B 细胞增殖减少、白细胞失衡以及体液和细胞免疫紊乱。主成分分析显示，体液免疫相关指标的相关性更大，CTX+PAMK 组与 CTX 组的差异最大，但与对照组接近。综上所述，PAMK 缓解了 CTX 引起的鹅的免疫抑制，对体液免疫的保护作用更明显且稳定。
