Department of Pharmacology, Guangdong Medical College, Dongguan, Guangdong 523808, P.R. China.
Mol Med Rep. 2013 Aug;8(2):463-7. doi: 10.3892/mmr.2013.1540. Epub 2013 Jun 21.
Protein‑coding genes and small non‑coding microRNAs involved in the guidance of differentiation in mesenchymal stem cells (MSCs) into osteoblasts have been extensively investigated in previous studies. However, long non‑coding RNAs (lncRNAs), which account for a large proportion of the genomic sequences in numerous species, have not yet been reported. In the present study, the lncRNA expression profile was analyzed using the Arraystar lncRNA array in C3H10T1/2 MSCs undergoing early osteoblast differentiation and 116 differentially expressed lncRNAs were identified between BMP‑2 treated and untreated groups. Among these lncRNAs, 59 were upregulated and 57 were downregulated in BMP‑2 treated groups. In addition, 24 cooperatively differentially expressed lncRNAs and nearby mRNA pairs were found. For example, mouselincRNA0231 and its nearby gene, EGFR, were downregulated, while lncRNA NR_027652 and its nearby gene, DLK1, were upregulated. These observations may be part of the regulatory mechanisms of lncRNAs in the control of osteoblast differentiaton. In conclusion, results of the present study indicate that lncRNA expression profiles are significantly altered in C3H10T1/2 undergoing early osteoblast differentiation and these results may provide insight into the mechanisms responsible for osteoblast differentiation.
先前的研究已经广泛探讨了参与间充质干细胞（MSCs）向成骨细胞分化的蛋白编码基因和小非编码 microRNAs。然而，在许多物种的基因组序列中占很大比例的长非编码 RNA（lncRNA）尚未得到报道。在本研究中，使用 Arraystar lncRNA 芯片分析了 C3H10T1/2 MSC 早期成骨分化过程中的 lncRNA 表达谱，在 BMP-2 处理和未处理组之间鉴定出 116 个差异表达的 lncRNA。在这些 lncRNA 中，BMP-2 处理组中有 59 个上调，57 个下调。此外，还发现了 24 个协同差异表达的 lncRNA 和附近的 mRNA 对。例如，mouselincRNA0231 及其附近基因 EGFR 下调，而 lncRNA NR_027652 及其附近基因 DLK1 上调。这些观察结果可能是 lncRNA 调控成骨细胞分化的机制的一部分。总之，本研究的结果表明，C3H10T1/2 在早期成骨分化过程中 lncRNA 的表达谱发生了显著改变，这些结果可能为成骨细胞分化的机制提供了新的认识。
