The Key Laboratory of Aerospace Medicine, Ministry of Education, The Fourth Military Medical University, Xi'an, Shaanxi 710032, P.R. China.
Mol Med Rep. 2017 Dec;16(6):8180-8188. doi: 10.3892/mmr.2017.7671. Epub 2017 Sep 29.
Long noncoding RNAs (lncRNAs) have been regarded as important regulators in numerous biological processes during cell development. However, the holistic lncRNA expression pattern and potential functions during osteoblast differentiation under simulated microgravity remain unknown. In the present study, a high throughput microarray assay was performed to detect lncRNA and mRNA expression profiles during MC3TC‑E1 pre‑osteoblast cell osteo‑differentiation under simulated microgravity. The expression of 857 lncRNAs and 2,264 mRNAs was signi-ficantly altered when MC3T3‑E1 cells were exposed to simulated microgravity. A relatively consistent distribution pattern on the chromosome and a co‑expression network were observed between the differentially‑expressed lncRNAs and mRNAs. Genomic context analysis further identified 132 differentially‑expressed lncRNAs and nearby coding gene pairs. Subsequently, 3 lncRNAs were screened out for their possible function in osteoblast differentiation, based on their co‑expression association and potential cis‑acting regulatory pattern with the deregulated mRNAs. The present study aimed to provide a comprehensive understanding of and a foundation for future studies into lncRNA function in mechanical signal‑mediated osteoblast differentiation.
长链非编码 RNA（lncRNA）已被认为是细胞发育过程中许多生物学过程中的重要调控因子。然而，在模拟微重力条件下成骨细胞分化过程中 lncRNA 的整体表达模式和潜在功能尚不清楚。本研究采用高通量微阵列分析检测了模拟微重力条件下 MC3TC-E1 前成骨细胞成骨分化过程中 lncRNA 和 mRNA 的表达谱。当 MC3T3-E1 细胞暴露于模拟微重力时，857 个 lncRNA 和 2264 个 mRNA 的表达明显改变。差异表达的 lncRNA 和 mRNA 之间观察到相对一致的染色体分布模式和共表达网络。基因组背景分析进一步鉴定了 132 个差异表达的 lncRNA 和附近的编码基因对。随后，根据共表达关联和与失调 mRNA 的潜在顺式作用调节模式，从这些差异表达的 lncRNA 中筛选出 3 个可能在成骨细胞分化中发挥作用的 lncRNA。本研究旨在为 lncRNA 在机械信号介导的成骨细胞分化中的功能提供全面的理解和未来研究的基础。
