Gerlach J, Vienken J, Walker P, Affeld K
Chirurgische Klinik, Freie Universität Berlin, F.R.G.
Int J Artif Organs. 1990 Jun;13(6):365-9.
An investigation was performed to demonstrate that time-lapse cinematography and computer aided video analysis of cell morphology is suitable to study and compare the characteristics of hepatocytes during the adhesion process to membranes. We chose to compare ordinary cellulose Cuprophan membranes and membranes coated with collagen or fibronectin. Striking differences between uncoated cellulose and fibronectin or collagen coating were seen in the cell count per square millimeter and adhesion behaviour. On the investigated uncoated Cuprophan the hepatocytes were found to attach but not to spread whilst on collagen coated Cuprophan most of the cells spread spherically, and on fibronectin coated membranes most of the cells flattened spherically or polygonally. Time-lapse video microscopy seems to be a valuable technique for assessing the morphologic behaviour of cells in a detailed and quantitative manner in order to improve the hepatocyte culture technique in bioreactors for hybrid systems.
进行了一项研究，以证明延时摄影和细胞形态的计算机辅助视频分析适用于研究和比较肝细胞在与膜粘附过程中的特征。我们选择比较普通的纤维素铜氨膜以及涂有胶原蛋白或纤连蛋白的膜。在每平方毫米的细胞计数和粘附行为方面，未涂层的纤维素与纤连蛋白或胶原蛋白涂层之间存在显著差异。在所研究的未涂层铜氨膜上，发现肝细胞能够附着但不会铺展，而在涂有胶原蛋白的铜氨膜上，大多数细胞呈球形铺展，在涂有纤连蛋白的膜上，大多数细胞呈球形或多边形扁平铺展。延时视频显微镜似乎是一种有价值的技术，可用于详细和定量地评估细胞的形态行为，以便改进用于混合系统的生物反应器中的肝细胞培养技术。
