Jóźwiak A, Karlik W, Wiechetek M, Weryński A
Institute of Biocybernetics and Biomedical Engineering PAS, Warsaw, Poland.
Int J Artif Organs. 1998 Aug;21(8):460-6.
Plasma or hormones added to hepatocyte incubation media mask the function of membranes as substrata per se for hepatocyte adhesion. This hypothesis was verified with hepatocyte cultures on various membranes in serum and hormone free medium. Freshly isolated rat hepatocytes were seeded on flat sheet membranes made of Cellulose Acetate (CA), aminated Cellulose Acetate (aCA), polysulfone (PSf) and sulfonated polysulfone (sPSf) and incubated in Hank's Balanced Salts Solutions (HBSS) as well as in William's E medium supplemented with newborn calf serum. It was found that PSf promoted hepatocyte adhesion most effectively. Good properties of PSf as a biomaterial for hepatocyte culture were confirmed in both media cultures. Urea synthesis and ammonia utilization measured in hepatocytes cultured on PSf were higher compared with other membrane cultures. PSf secured longer viability for a higher number of cells seeded on membrane compared with other investigated membranes, which is the reason for higher metabolic activity in PSf culture.
添加到肝细胞培养介质中的血浆或激素会掩盖膜本身作为肝细胞黏附底物的功能。该假设在无血清和无激素培养基中于各种膜上进行的肝细胞培养实验中得到了验证。将新鲜分离的大鼠肝细胞接种在由醋酸纤维素（CA）、胺化醋酸纤维素（aCA）、聚砜（PSf）和磺化聚砜（sPSf）制成的平板膜上，并在汉克平衡盐溶液（HBSS）以及添加了新生牛血清的威廉姆斯E培养基中培养。结果发现，聚砜最有效地促进了肝细胞黏附。在两种培养基培养中均证实了聚砜作为肝细胞培养生物材料的良好性能。与其他膜培养相比，在聚砜上培养的肝细胞中测得的尿素合成和氨利用更高。与其他研究的膜相比，聚砜能确保接种在膜上的更多细胞具有更长的活力，这就是聚砜培养中代谢活性更高的原因。
