Gerlach J, Klöppel K, Schauwecker H H, Tauber R, Müller C, Bücherl E S
Chirurgische Klinik, Freie Universität Berlin, West Germany.
Int J Artif Organs. 1989 Dec;12(12):788-92.
Hepatocyte cultivation in bioreactors for hybrid liver support systems is possible under two conditions: attached to a substrate like membranes or microcarriers or in suspension culture. To compare the ammonia metabolism of hepatocytes cultivated under these two conditions, cultures of primary seeded rat hepatocytes were cultivated either attached to collagen coated tissue culture plastic or as a suspension culture. During the time course of culture, the ability of hepatocytes to reduce the ammonia content of the medium decreased in both adhesion and suspension cultures, though to different extents. In suspension cultures, ammonia content was reduced from 350 microM to about 100 microM (day 4) and to about 180 microM (day 6). No significant reduction was seen on day 8 of culture. In contrast, hepatocytes attached to collagen coated dishes remained viable and functional for at least 8 days after plating, reducing ammonia content from 350 microM to 70 microM (day 4), 90 microM (day 6) and 180 microM (day 8). The period of useful metabolism of hepatocytes in bioreactors for hybrid liver support systems appears to depend on the culture conditions.
附着于诸如膜或微载体等基质上，或进行悬浮培养。为比较在这两种条件下培养的肝细胞的氨代谢情况，将原代接种的大鼠肝细胞培养物分别附着于胶原包被的组织培养塑料上或进行悬浮培养。在培养过程中，肝细胞降低培养基中氨含量的能力在贴壁培养和悬浮培养中均有所下降，不过程度不同。在悬浮培养中，氨含量从350微摩尔/升降至约100微摩尔/升（第4天）和约180微摩尔/升（第6天）。培养第8天未观察到显著降低。相比之下，附着于胶原包被培养皿的肝细胞在接种后至少8天仍保持活力和功能，氨含量从350微摩尔/升降至70微摩尔/升（第4天）、90微摩尔/升（第6天）和180微摩尔/升（第8天）。用于混合肝支持系统的生物反应器中肝细胞有效代谢的时间似乎取决于培养条件。
