Crop Research Institute, Drnovska 507, 161 06 Prague 6 Ruzyne, Czech Republic.
Chemosphere. 2013 Oct;93(6):1051-6. doi: 10.1016/j.chemosphere.2013.05.076. Epub 2013 Jun 22.
In terms of food safety, species of the Fusarium, Aspergillus and Penicillium genera are considered the most significant because they produce the great majority of known mycotoxins. Developing resistance against commonly used fungicides have become a critical problem in area such as agriculture, the storage and production of food and even in human medicines. The need for research and development of new alternative antifungal treatment based on natural antifungal substances is obvious. Here, the antifungal efficacy of 21 phenolic components of essential oils and plant substances were tested against these filamentous fungi with respect to their different molecular structures. Minimum inhibitory concentration values MIC₅₀ and MIC₁₀₀ were successfully estimated for 15 substances by means of probit analysis. Thymol and carvacrol were evaluated as the most effective. The MIC₅₀ values for thymol ranged from 30 to 52 μg mL(-1). The MIC₁₀₀ values for thymol ranged from 76 to 255 μg mL(-1), respectively. For carvacrol, the MIC₅₀ values ranged from 37 to 76 μg mL(-1), and the MIC100 ranged from 131 to 262 μg mL(-1). The results also revealed differences in the efficacy of phenols depending on molecular structures and different inter-species sensitivity.
就食品安全而言，镰刀菌属、曲霉属和青霉属的物种被认为是最重要的，因为它们产生了绝大多数已知的霉菌毒素。在农业、食品储存和生产，甚至人类医药等领域，对常用杀真菌剂产生抗性已成为一个关键问题。显然，需要研究和开发基于天然抗真菌物质的新的替代抗真菌治疗方法。在这里，根据不同的分子结构，测试了 21 种精油和植物物质中的酚类成分对这些丝状真菌的抗真菌功效。通过概率分析成功估计了 15 种物质的最小抑菌浓度值 MIC₅₀ 和 MIC₁₀₀。百里酚和香芹酚被评估为最有效的。百里酚的 MIC₅₀ 值范围为 30 至 52 μg mL(-1)。百里酚的 MIC₁₀₀ 值范围为 76 至 255 μg mL(-1)。对于香芹酚，MIC₅₀ 值范围为 37 至 76 μg mL(-1)，MIC₁₀₀ 值范围为 131 至 262 μg mL(-1)。结果还表明，根据分子结构和不同种间敏感性的不同，酚类化合物的功效存在差异。
