Department of Botany, School of Biology, Aristotle University of Thessaloniki, GR-541 24 Thessaloniki, Greece.
Chemosphere. 2013 Oct;93(6):1035-42. doi: 10.1016/j.chemosphere.2013.05.074. Epub 2013 Jun 22.
The effects of increasing Cu, Ni and Cr concentrations (0.5, 5, 10, 20 and 40 mg L(-1)) on microtubule organization and the viability of leaf cells of the seagrass Cymodocea nodosa for 13 consecutive days were investigated under laboratory conditions. Increased oblique microtubule orientation, microtubule depolymerization at the 5-40 mg L(-1) Ni treatments after 3 d of exposure, and a complete microtubule depolymerization at all Ni treatments after 5 d were observed. Cu depolymerised microtubules after three to 7 d of exposure, while Cr caused an extensive microtubule bundling after 9 or 11 d of exposure, depending on metal dosage. Fluorescence intensity measurements further consolidated the above phenomena. Cell death, occurring at later time than microtubule disturbance, was also observed at all Cu and Ni treatments and at the 10-40 mg L(-1) Cr treatments and adding to the above quantification of the number of dead cells clearly showed that only a portion of the cell population studied died. The data presented, being the first assessment of microtubule disturbance in seagrasses, indicate that microtubules in seagrass leaf cells could be used as a valuable and early marker of metal-induced stress in biomonitoring programmes.
在实验室条件下，研究了连续 13 天增加 Cu、Ni 和 Cr 浓度（0.5、5、10、20 和 40mg/L）对海洋草（Cymodocea nodosa）叶片细胞微管组织和活力的影响。暴露 3 天后，在 5-40mg/L Ni 处理下观察到斜向微管取向增加，微管解聚，在所有 Ni 处理下 5 天后观察到完全微管解聚。Cu 在暴露 3-7 天后解聚微管，而 Cr 在暴露 9-11 天后根据金属剂量导致广泛的微管束。荧光强度测量进一步证实了上述现象。细胞死亡发生在微管紊乱之后，在所有 Cu 和 Ni 处理以及 10-40mg/L Cr 处理中都观察到，并且对死亡细胞数量的上述定量分析表明，只有部分研究细胞群死亡。所提出的数据首次评估了海洋草中的微管紊乱，表明海洋草叶片细胞中的微管可用作生物监测计划中金属诱导应激的有价值和早期标志物。
