组织制备的微生物检测使用 BACTEC™ 血培养系统的验证。
Validation of the Microbiological Testing of Tissue Preparations Using the BACTEC™ Blood Culture System.
机构信息
University Tissue Bank, Cornea Bank Berlin, Institute of Transfusion Medicine, Charité - Universitätsmedizin Berlin, Germany.
出版信息
Transfus Med Hemother. 2012 Dec;39(6):387-90. doi: 10.1159/000345812. Epub 2012 Nov 21.
BACKGROUND
Since blood culture bottles are validated by the manufacturer for blood only, an additional validation for the use with fluids of tissue preparations is necessary.
METHODS
Two 10-ml samples of cornea culture medium, histidine-tryptophan-ketoglutarate (HTK) solution, or Ringer solution at the end of femur head thermo-disinfection were given into blood culture bottles (BD BACTEC™ Plus Aerobic/F, Anaerobic/F for cornea culture medium and BD BACTEC™ Standard Aerobic/Anaerobic for HTK and Ringer solution) and subsequently spiked with 10-100 colony forming units (CFU) of bacteria or fungi (aerobic bacteria: Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa; anaerobic bacteria: Clostridium sporogenes; fungi: Candida albicans, Aspergillus brasiliensis) according to the European Pharmacopoeia Chapter 2.6.1.
RESULTS
All tested bacteria and fungi could be detected in all solutions. All positive and negative controls were tested correctly. Compared to the positive controls, the microbial growth was delayed in the antibiotic-containing cornea culture medium, and negative in two cases of B. subtilis spiking.
CONCLUSION
The use of BACTEC™ blood culture bottles seems to be a suitable method for microbiological testing of HTK solution, Ringer solution, and, with limitations, also for testing of the antibiotic-containing cornea culture medium.
背景
由于血培养瓶是由制造商专门用于血液的，因此需要对其与组织准备液的使用进行额外的验证。
方法
在股骨头部热消毒结束时，将两份 10ml 的角膜培养液、组氨酸-色氨酸-酮戊二酸（HTK）溶液或林格氏溶液分别注入血培养瓶（BD BACTEC™ Plus 需氧/厌氧瓶，用于角膜培养液；BD BACTEC™ Standard 需氧/厌氧瓶，用于 HTK 和林格氏溶液），然后用 10-100 个菌落形成单位（CFU）的细菌或真菌（需氧菌：金黄色葡萄球菌、枯草芽孢杆菌、铜绿假单胞菌；厌氧菌：梭状芽孢杆菌；真菌：白色念珠菌、巴西曲霉）对其进行接种，接种量根据欧洲药典第 2.6.1 章的要求进行。
结果
所有测试的细菌和真菌都能在所有溶液中被检测到。所有阳性和阴性对照都被正确地检测到。与阳性对照相比，含有抗生素的角膜培养液中的微生物生长延迟，枯草芽孢杆菌的接种结果为阴性。
结论
使用 BACTEC™ 血培养瓶似乎是一种适用于 HTK 溶液、林格氏溶液微生物检测的方法，对于含有抗生素的角膜培养液的检测，也有一定的局限性。
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