Paulan Silvana de Cássia, Gonzáles Rutilia Marisela Hernándes, Peralta Laura Adalid, Vicentini-Oliveira Josy Campanhã, Biondi Germano Francisco, Conde Edda Sciuto, Parkhouse Robert Michael Evans, Nunes Cáris Maroni
Departamento de Apoio, Produção e Saúde Animal, Universidade Estadual Paulista Júlio de Mesquita Filho, Araçatuba, SP, Brazil.
Rev Bras Parasitol Vet. 2013 Apr-Jun;22(2):270-5. doi: 10.1590/S1984-29612013005000026.
Bovine cysticercosis, a cosmopolitan disease caused by Taenia saginata, leads to economic losses due to carcass devaluation at slaughter. Sanitary inspection at slaughterhouses, the routine diagnostic method in Brazil, lacks the necessary sensitivity to detect the mildly infected cattle that are typically encoutered in Brazil. In this study we have tested cattle sera from animals diagnosed as positive and negative by veterianry inspection for (1) anti-parasite antibodies using metacestodes antigens (T. solium vesicular fluid and T. saginata secretions) and (2) the HP10 secreted antigen of viable metacestodes. The cut-off values were calculated by ROC curve for intense and mild infections conditions, and by the classical method ( for negative samples). The sensitivity and specificity of these diagnostic tests were different depending on the assumed cut-off value and, importantly, whether the infection was mild or intense. In spite of these observations, however, such ELISA assays for serum antibodies and parasite antigens constitute an important tool for epidemiological porposes, and in establishing priorities for the control of bovine cysticercosis.
牛囊尾蚴病是一种由牛带绦虫引起的世界性疾病，由于屠宰时胴体贬值而导致经济损失。巴西的常规诊断方法是在屠宰场进行卫生检查，但这种方法缺乏必要的敏感性，无法检测出巴西常见的轻度感染牛。在本研究中，我们检测了经兽医检查诊断为阳性和阴性的牛血清，以检测：（1）使用囊尾蚴抗原（猪带绦虫囊泡液和牛带绦虫分泌物）检测抗寄生虫抗体；（2）检测活囊尾蚴分泌的HP10抗原。通过ROC曲线计算重度和轻度感染情况下的临界值，并通过经典方法（针对阴性样本）计算临界值。这些诊断测试的敏感性和特异性因假定的临界值不同而不同，重要的是，还取决于感染是轻度还是重度。然而，尽管有这些观察结果，但这种针对血清抗体和寄生虫抗原的ELISA检测方法仍是流行病学研究的重要工具，对于确定牛囊尾蚴病的控制重点也很重要。
