SMARTS, Metallurgy and Materials Group, Indira Gandhi Centre for Atomic Research, Kalpakkam 603102, India.
J Biomed Nanotechnol. 2013 May;9(5):751-64. doi: 10.1166/jbn.2013.1590.
We present methodologies to functionalize iron oxide (Fe3O4) nanoparticles with biosurfactants and biocompatibility results. Positively charged Fe3O4 nanoparticles of average hydrodynamic size -26 nm is functionalized with four different molecules of interest, viz., surfactin, rhamnolipid, polyethylene glycol (PEG) and dextran. The functionalization results in dramatic alterations in surface potential and hydrodynamic size due to the presence of coated moieties on the nanoparticle interface. The Fourier transform infrared spectroscopy and thermogravimetric analysis confirm the presence of adsorbed moieties on nanoparticles. The phase contrast microscopy studies show the formation of reversible chains of functionalized nanoparticles under an external magnetic field. Cell viability studies using L929 mouse fibroblast cell line show that pure surfactin, rhamnolipid and dextran exhibit cytotoxicity with increase in concentration, whereas, pure PEG exhibit biocompatibility at different concentrations. Accordingly, surfactin and rhamnolipid coated nanoparticles are found to be cytotoxic with increase in concentration and PEG coated nanoparticles are found to be biocompatible. Dextran coated nanoparticles do not exhibit significant increase in biocompatibility.
我们提出了用生物表面活性剂功能化氧化铁（Fe3O4）纳米粒子的方法，并得到了生物相容性的结果。平均水动力直径为-26nm 的带正电荷的 Fe3O4 纳米粒子用四种不同的感兴趣的分子进行了功能化，即表面活性剂、鼠李糖脂、聚乙二醇（PEG）和葡聚糖。由于纳米粒子界面上存在涂层部分，功能化导致表面电位和水动力尺寸发生显著变化。傅里叶变换红外光谱和热重分析证实了吸附在纳米粒子上的部分的存在。相差显微镜研究表明，在外磁场下，功能化纳米粒子形成了可逆的链。使用 L929 小鼠成纤维细胞系进行的细胞活力研究表明，纯表面活性剂、鼠李糖脂和葡聚糖的浓度增加时表现出细胞毒性，而不同浓度的纯 PEG 表现出生物相容性。因此，随着浓度的增加，表面活性剂和鼠李糖脂包覆的纳米粒子被发现具有细胞毒性，而 PEG 包覆的纳米粒子被发现具有生物相容性。葡聚糖包覆的纳米粒子没有表现出明显的生物相容性增加。
