Department of Medical Nanobiotechnology, Pirogov Russian National Research Medical University, Moscow, Russian Federation.
Laboratory of Biomedical Nanomaterials, National University of Science and Technology MISiS, Moscow, Russian Federation.
J Biochem Mol Toxicol. 2018 Dec;32(12):e22225. doi: 10.1002/jbt.22225. Epub 2018 Oct 5.
Toxicological research of novel nanomaterials is a major developmental step of their clinical approval. Since iron oxide magnetic nanoparticles have a great potential in cancer treatment and diagnostics, the investigation of their toxic properties is very topical. In this paper we synthesized bovine serum albumin-coated iron oxide nanoparticles with different sizes and their polyethylene glycol derivative. To prove high biocompatibility of obtained nanoparticles the number of in vitro toxicological tests on human fibroblasts and U251 glioblastoma cells was performed. It was shown that albumin nanoparticles' coating provides a stable and biocompatible shell and prevents cytotoxicity of magnetite core. On long exposure times (48 hours), cytotoxicity of iron oxide nanoparticles takes place due to free radical production, but this toxic effect may be neutralized by using polyethylene glycol modification.
新型纳米材料的毒理学研究是其临床批准的重要发展步骤。由于氧化铁磁性纳米颗粒在癌症治疗和诊断方面具有巨大的潜力，因此对其毒性特性的研究非常热门。在本文中，我们合成了具有不同粒径的牛血清白蛋白包覆的氧化铁纳米颗粒及其聚乙二醇衍生物。为了证明所获得的纳米颗粒具有高的生物相容性，对人成纤维细胞和 U251 神经胶质瘤细胞进行了多种体外毒理学试验。结果表明，白蛋白纳米颗粒的涂层提供了稳定且生物相容的外壳，并防止了磁铁矿核心的细胞毒性。在较长的暴露时间（48 小时）下，由于自由基的产生，氧化铁纳米颗粒会发生细胞毒性，但这种毒性作用可以通过聚乙二醇修饰来中和。
