Michiels C, Raes M, Remacle J
Arch Int Physiol Biochim. 1986 Dec;94(5):S13-7.
An experimental model was developed in order to study the protective effect of antioxidant molecules. Human diploid WI-38 fibroblasts were cultivated under 2 atm of 95% O2. Antioxidants like alpha-tocopherol or superoxide dismutase (SOD) were added respectively in the culture medium or directly inside the cell through a microinjection technique. With both antioxidant molecules a protection was observed. In the control experiment, cells died within 6 or 8 days depending on the confluency and the malonaldehyde content increased sharply. This model represents a new tool in order to test other antioxidant systems towards an oxidative stress.
为了研究抗氧化分子的保护作用，建立了一个实验模型。人二倍体WI-38成纤维细胞在95%氧气的2个大气压下培养。抗氧化剂如α-生育酚或超氧化物歧化酶（SOD）分别添加到培养基中或通过显微注射技术直接注入细胞内。使用这两种抗氧化分子均观察到了保护作用。在对照实验中，细胞根据汇合度在6或8天内死亡，丙二醛含量急剧增加。该模型是一种用于测试其他抗氧化系统应对氧化应激的新工具。
