Le Clec'h Winka, Raimond Maryline, Guillot Sylvain, Bouchon Didier, Sicard Mathieu
Université de Poitiers, Laboratoire Écologie et Biologie des Interactions, UMR CNRS 7267, Équipe Écologie Évolution Symbiose, 40 avenue du recteur Pineau, F-86022 Poitiers cedex, France.
Université de Poitiers, Laboratoire Écologie et Biologie des Interactions, UMR CNRS 7267, Équipe Écologie Évolution Symbiose, 40 avenue du recteur Pineau, F-86022, Poitiers cedex, France.
Environ Microbiol. 2013 Nov;15(11):2922-36. doi: 10.1111/1462-2920.12172. Epub 2013 Jun 27.
The endosymbiont Wolbachia pipientis infects various hosts in which it navigates vertically from mothers to offspring. However, horizontal transfers of Wolbachia can occur between hosts. The virulence of the horizontally acquired Wolbachia can change in the new host as it has been illustrated by the case of the feminizing strain wVulC from the woodlouse Armadillidium vulgare that turns to a pathogen when introduced into Porcellio dilatatus dilatatus. In the present study, we aim to show whether symbiotic traits, such as (i) host sex manipulation and (ii) colonization patterns, which differ between eight isopod Wolbachia strains, are connected to their virulence towards the recipient host P. d. dilatatus. Among the transferred Wolbachia, some feminizing strains gradually differing in feminizing intensity in their native hosts induced different levels of pathogenicity to P. d. dilatatus. Not a single feminizing strain passed vertically with high titres to the next generation. The non-feminizing Wolbachia strains, even if they reached high densities in the host, did not impact host life-history traits and some vertically passed with high titres to the offspring. These results suggest that a potential link between the manners Wolbachia manipulates its native host reproduction, its virulence and its ability to vertically infect the offspring.
内共生菌沃尔巴克氏体（Wolbachia pipientis）感染多种宿主，并通过垂直传播从母体传递给后代。然而，沃尔巴克氏体也可在宿主之间发生水平转移。正如从普通卷甲虫（Armadillidium vulgare）分离出的雌性化菌株wVulC，当被引入到膨腹体（Porcellio dilatatus dilatatus）时变成病原体，这一案例表明，水平获得的沃尔巴克氏体在新宿主中的毒力可能会发生变化。在本研究中，我们旨在探究8种等足目沃尔巴克氏体菌株之间存在差异的共生特性，如（i）宿主性别操控和（ii）定殖模式，是否与它们对受体宿主膨腹体的毒力相关。在转移的沃尔巴克氏体中，一些在其原生宿主中雌性化强度逐渐不同的雌性化菌株，对膨腹体表现出不同程度的致病性。没有一个雌性化菌株能以高滴度垂直传递给下一代。非雌性化的沃尔巴克氏体菌株，即使在宿主体内达到高密度，也不会影响宿主的生活史特征，并且一些菌株能以高滴度垂直传递给后代。这些结果表明，沃尔巴克氏体操控其原生宿主繁殖的方式、其毒力以及垂直感染后代的能力之间可能存在联系。
