Sicard Mathieu, Bouchon Didier, Ceyrac Laura, Raimond Roland, Thierry Magali, Le Clec'h Winka, Marcadé Isabelle, Caubet Yves, Grève Pierre
Université de Poitiers - UFR Sciences Fondamentales et Appliquées, Laboratoire EBI Ecologie & Biologie des Interactions, UMR CNRS 7267, Équipe Ecologie, Evolution, Symbiose, Bât. B8-B35, 5, rue Albert Turpin, TSA 51106, F-86073 Poitiers Cedex 9, France.
Université de Poitiers - UFR Sciences Fondamentales et Appliquées, Laboratoire EBI Ecologie & Biologie des Interactions, UMR CNRS 7267, Équipe Ecologie, Evolution, Symbiose, Bât. B8-B35, 5, rue Albert Turpin, TSA 51106, F-86073 Poitiers Cedex 9, France.
J Invertebr Pathol. 2014 Sep;121:28-36. doi: 10.1016/j.jip.2014.06.007. Epub 2014 Jun 28.
In the terrestrial isopod species Porcellio dilatatus, unidirectional Cytoplasmic Incompatibility (CI) between two morphs (P. d. dilatatus and P. d. petiti) caused by a Wolbachia strain (wPet) infecting the morph P. d. petiti has been previously described by experiments initiated four decades ago. Here, we studied another Wolbachia that has been recently detected in a population of the morph P. d. dilatatus. The MLST markers reveal that this Wolbachia is a new strain called wDil distinct from wPet also belonging to the isopod clade of Wolbachia. Quantifications of both Wolbachia strains in the gonads of the two P. dilatatus morphs revealed that all males exhibit similar Wolbachia titers while the titers in females depend on the Wolbachia strain they host. Crossing experiments showed that both wDil and wPet induced partial unidirectional CI with different intensities. Moreover, these two strains induced bidirectional CI when individuals were both infected with one of the two different Wolbachia strains. This way, we demonstrated that P. dilatatus can be infected by two closely related Wolbachia strains (wDil and wPet), that seem to have different modification-rescue systems.
在陆生等足类动物物种膨腹土甲（Porcellio dilatatus）中，由感染膨腹土甲（P. d. petiti）形态的一种沃尔巴克氏体菌株（wPet）引起的两种形态（膨腹土甲指名亚种P. d. dilatatus和膨腹土甲佩氏亚种P. d. petiti）之间的单向细胞质不亲和性（CI），早在四十年前启动的实验中就已被描述。在此，我们研究了最近在膨腹土甲指名亚种群体中检测到的另一种沃尔巴克氏体。多位点序列分型（MLST）标记显示，这种沃尔巴克氏体是一种名为wDil的新菌株，与同样属于沃尔巴克氏体等足类进化枝的wPet不同。对膨腹土甲两种形态的性腺中两种沃尔巴克氏体菌株的定量分析表明，所有雄性的沃尔巴克氏体滴度相似，而雌性的滴度则取决于它们所携带的沃尔巴克氏体菌株。杂交实验表明，wDil和wPet都会诱导不同强度的部分单向细胞质不亲和性。此外，当个体同时感染两种不同沃尔巴克氏体菌株中的一种时，这两种菌株会诱导双向细胞质不亲和性。通过这种方式，我们证明了膨腹土甲可以被两种密切相关的沃尔巴克氏体菌株（wDil和wPet）感染，这两种菌株似乎具有不同的修饰挽救系统。
