膜近端“茎”区增加了黄病毒 E 蛋白融合后三聚体的稳定性，并调节其结构。
The membrane-proximal "stem" region increases the stability of the flavivirus E protein postfusion trimer and modulates its structure.
机构信息
Department of Virology, Medical University of Vienna, Vienna, Austria.
出版信息
J Virol. 2013 Sep;87(17):9933-8. doi: 10.1128/JVI.01283-13. Epub 2013 Jun 26.
Abstract
The flavivirus fusion protein E contains a "stem" region which is hypothesized to be crucial for driving fusion. This sequence element connects the ectodomain to the membrane anchor, and its structure in the trimeric postfusion conformation is still poorly defined. Using E trimers of tick-borne encephalitis virus with stem truncations of different lengths, we show that the N-terminal part of the stem increases trimer stability and also modulates the trimer structure outside the stem interaction site.
摘要
黄病毒融合蛋白 E 包含一个“茎”区，该区域被假设对于驱动融合至关重要。该序列元件将外域连接到膜锚定，但其在三聚体融合构象中的结构仍未得到很好的定义。使用具有不同长度茎截断的蜱传脑炎病毒 E 三聚体，我们表明茎的 N 端部分增加了三聚体稳定性，并且还调节了茎相互作用位点之外的三聚体结构。
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