Markina O V, Alekseeva L P, Markin N V, Telesmanich N R
Zh Mikrobiol Epidemiol Immunobiol. 2013 Jan-Feb(1):9-13.
Study the activity of plant extracts against cholera toxin (CT) of Vibrio cholerae O1.
Antitoxic activity of plant extracts was determined by using enzyme immunoassay and CHO-K1 cell culture.
8 water extracts of plants were studied. Extracts of nut, tutsan, milfoil, basil do not have effect on CT activity in EIA or CHO-K1 cell culture. Celandine and rhubarb extracts do not reduce CT immunochemical activity but prevent elongation of CHO-K1 cells. Oak and hop extracts suppress binding in EIA of cholera toxin and GM1 receptors and insignificantly reduce its activity in cell culture.
Antitoxic activityofplant extracts against CT is perspective for the development of preparations possessing inhibition effect.
研究植物提取物对霍乱弧菌O1型霍乱毒素（CT）的活性。
采用酶免疫测定法和CHO-K1细胞培养法测定植物提取物的抗毒素活性。
研究了8种植物的水提取物。坚果、土三七、蓍草、罗勒的提取物对酶免疫测定法或CHO-K1细胞培养中的CT活性无影响。白屈菜和大黄提取物不会降低CT免疫化学活性，但可阻止CHO-K1细胞伸长。橡木和啤酒花提取物可抑制霍乱毒素与GM1受体在酶免疫测定法中的结合，并在细胞培养中使其活性略有降低。
植物提取物对CT的抗毒素活性对于开发具有抑制作用的制剂具有前景。
