GenVec, Inc., 65W. Watkins Mill Rd, Gaithersburg, MD 20878, USA.
Virology. 2013 Sep;444(1-2):119-23. doi: 10.1016/j.virol.2013.05.041. Epub 2013 Jun 24.
We have isolated and cultured three distinct adenoviruses from wild gorillas. Phylogenetic analysis grouped the viruses with human adenovirus species C based on DNA polymerase, hexon, and E4ORF6 genes. The three wild gorilla adenoviruses clustered with the other species C captive gorilla adenoviruses, forming a branch separate from human and chimpanzee/bonobo adenoviruses. Animal sera to the three newly isolated viruses did not cross-neutralize, demonstrating serological distinctiveness. The human adenovirus 5 fiber knob blocked infection, suggesting use of the Coxsackie and Adenovirus Receptor. These viruses may provide viral vectors with properties distinct from chimpanzee adenovirus and human adenovirus vectors.
我们从野生大猩猩中分离和培养了三种不同的腺病毒。基于 DNA 聚合酶、六邻体和 E4ORF6 基因的系统发育分析将这些病毒归为人类腺病毒 C 种。三种野生大猩猩腺病毒与其他圈养大猩猩腺病毒 C 种聚在一起，形成一个与人类和黑猩猩/倭黑猩猩腺病毒分开的分支。三种新分离的病毒的动物血清没有交叉中和，表明血清学上具有独特性。人腺病毒 5 纤维结阻止了感染，提示使用柯萨奇病毒和腺病毒受体。这些病毒可能提供与黑猩猩腺病毒和人腺病毒载体不同特性的病毒载体。
