感染濒危川金丝猴（Rhinopithecus roxellana）的腺病毒的分离与鉴定
Isolation and characterization of adenoviruses infecting endangered golden snub-nosed monkeys (Rhinopithecus roxellana).
作者信息
Tan Bing, Wu Li-Jun, Yang Xing-Lou, Li Bei, Zhang Wei, Lei Yong-Song, Li Yong, Yang Guo-Xiang, Chen Jing, Chen Guang, Wang Han-Zhong, Shi Zheng-Li
机构信息
Key Laboratory of Special Pathogens and Center for Emerging Infectious Diseases, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China.
University of Chinese Academy of Sciences, Beijing, China.
出版信息
Virol J. 2016 Nov 25;13(1):190. doi: 10.1186/s12985-016-0648-6.
BACKGROUND
Adenoviruses are important pathogens with the potential for interspecies transmission between humans and non-human primates. Although many adenoviruses have been identified in monkeys, the knowledge of these viruses from the Colobinae members is quite limited.
FINDINGS
We conducted a surveillance of viral infection in endangered golden snub-nosed monkeys (Rhinopithecus roxellana) in the subfamily Colobinae in China, and found that 5.1% of sampled individuals were positive for adenovirus. One of the adenoviruses (SAdV-WIV19) was successfully isolated and its full-length genome was sequenced. The full-length genome of WIV19 is 33,562 bp in size, has a G + C content of 56.2%, and encodes 35 putative genes. Sequence analysis revealed that this virus represents a novel species in the genus Mastadenovirus. Diverse cell lines, including those of human origin, were susceptible to WIV19.
CONCLUSION
We report the first time the isolation and full-length genomic characterization of an adenovirus from the subfamily Colobinae.
背景
腺病毒是重要的病原体，具有在人类和非人灵长类动物之间跨物种传播的潜力。尽管在猴子中已鉴定出许多腺病毒，但关于叶猴亚科成员中这些病毒的了解相当有限。
研究结果
我们对中国叶猴亚科濒危川金丝猴（Rhinopithecus roxellana）的病毒感染情况进行了监测，发现5.1%的采样个体腺病毒呈阳性。其中一种腺病毒（SAdV-WIV19）被成功分离，并对其全长基因组进行了测序。WIV19的全长基因组大小为33,562 bp，G+C含量为56.2%，编码35个推定基因。序列分析表明，该病毒代表哺乳动物腺病毒属中的一个新物种。包括人源细胞系在内的多种细胞系对WIV19敏感。
结论
我们首次报告了从叶猴亚科分离出腺病毒并对其进行全长基因组特征分析。
Zotero 插件