Department of Immunology and Microbiology, Basic Medical College, Zhengzhou University, No.100 of Science Road, Zhengzhou, 450001, China.
Neurol Sci. 2014 Feb;35(2):191-7. doi: 10.1007/s10072-013-1476-8. Epub 2013 Jun 27.
The normal thymus contributes to T lymphocytes differentiation and induction of tolerance to self-antigens. Myasthenia gravis (MG) is characterized by abnormal thymic hyperplasia. To assess the potential influence of MG-thymus on the differentiation of T lymphocytes differentiation, we used the MG-thymus transplanted severe combined immunodeficiency (SCID) mice model to evaluate the human cord blood stem cells differentiation. Thymus fragments from MG patient and human cord blood stem cells were transplanted into SCID mice successively. SCID mice were observed to develop sustained human T lymphocytes and a functional anti-tumor immune. The levels of various T cell subsets in SCID mice with MG-thymus were different from that of control group. Among that, the frequency of CD4(+)CD25(+) T cells was significant lower in SCID mice with MG-thymus. The deficiency of CD4(+)CD25(+) T cells seens to contribute to the pathogenesis of MG.
正常的胸腺有助于 T 淋巴细胞的分化，并诱导对自身抗原的耐受。重症肌无力（MG）的特征是异常的胸腺增生。为了评估 MG 胸腺对 T 淋巴细胞分化的潜在影响，我们使用 MG 胸腺移植严重联合免疫缺陷（SCID）小鼠模型来评估人脐血干细胞的分化。从 MG 患者和人脐血干细胞中取出胸腺片段，先后移植到 SCID 小鼠体内。观察到 SCID 小鼠持续产生人类 T 淋巴细胞和具有功能的抗肿瘤免疫。MG 胸腺 SCID 小鼠中的各种 T 细胞亚群水平与对照组不同。其中，MG 胸腺 SCID 小鼠中 CD4(+)CD25(+)T 细胞的频率显著降低。CD4(+)CD25(+)T 细胞的缺乏似乎导致了 MG 的发病机制。
