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夸特氏毕赤酵母新种,基于多基因序列分析的毕赤酵母(巴斯德毕赤酵母属)新姊妹种。

Komagataella kurtzmanii sp. nov., a new sibling species of Komagataella (Pichia) pastoris based on multigene sequence analysis.

机构信息

State Institute for Genetics and Selection of Industrial Microorganisms, 1-Dorozhnyi 1, Moscow, Russia.

出版信息

Antonie Van Leeuwenhoek. 2013 Sep;104(3):339-47. doi: 10.1007/s10482-013-9956-7. Epub 2013 Jun 27.

Abstract

A novel methanol assimilating yeast species Komagataella kurtzmanii is described using the type strain VKPM Y-727 (=KBP Y-2878 = UCD-FST 76-20 = Starmer #75-208.2 = CBS 12817 = NRRL Y-63667) isolated by W.T. Starmer from a fir flux in the Catalina Mountains, Southern AZ, USA. The new species is registered in MycoBank under MB 803919. The species was differentiated by divergence in gene sequences for D1/D2 LSU rRNA, ITS1-5.8S-ITS2, RNA polymerase subunit I, translation elongation factor-1α and mitochondrial small subunit rRNA. K. kurtzmanii differs from its phenotypically similar sibling species Komagataella pastoris, Komagataella pseudopastoris, Komagataella phaffii, Komagataella populi and Komagataella ulmi by absence of growth at 35 °C and inability to assimilate trehalose.

摘要

一种新型甲醇同化酵母物种 Komagataella kurtzmanii 使用模式菌株 VKPM Y-727(= KBP Y-2878 = UCD-FST 76-20 = Starmer #75-208.2 = CBS 12817 = NRRL Y-63667)进行了描述,该菌株由 W.T. Starmer 从美国亚利桑那州南部卡特琳娜山脉的冷杉液流中分离得到。该新物种已在 MycoBank 中注册,注册号为 MB 803919。该物种通过 D1/D2 LSU rRNA、ITS1-5.8S-ITS2、RNA 聚合酶亚基 I、延伸因子-1α 和线粒体小亚基 rRNA 的基因序列差异来区分。K. kurtzmanii 与其表型相似的亲缘种 Komagataella pastoris、Komagataella pseudopastoris、Komagataella phaffii、Komagataella populi 和 Komagataella ulmi 不同,它不能在 35°C 下生长,也不能同化海藻糖。

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