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新种蒙氏毕赤酵母(Komagataella mondaviorum sp. nov.)的描述,它是巴斯德毕赤酵母(Komagataella (Pichia) pastoris)的一个新姊妹种。

Description of Komagataella mondaviorum sp. nov., a new sibling species of Komagataella (Pichia) pastoris.

作者信息

Naumov Gennadi I, Naumova Elena S, Boundy-Mills Kyria L

机构信息

State Research Institute of Genetics and Selection of Industrial Microorganisms of National Research Centre "Kurchatov Institute", Moscow, 117545, Russia.

Phaff Yeast Culture Collection, Department of Food Science and Technology, University of California, Davis, 95616, CA, USA.

出版信息

Antonie Van Leeuwenhoek. 2018 Jul;111(7):1197-1207. doi: 10.1007/s10482-018-1028-6. Epub 2018 Jan 31.

Abstract

Five methylotrophic strains (UCDFST 71-1024, UCDFST 54-11.16, UCDFST 54-11.141, UCDFST 68-967.1 and UCDFST 74-1030) from the Phaff Yeast Culture Collection (University of California Davis, USA) that were originally designated as Pichia pastoris were found to represent a novel Komagataella species. Strains of Komagataella mondaviorum sp. nov. UCDFST 71-1024(type strain) = CBS 15017, UCDFST 54-11.16, UCDFST 54-11.141, UCDFST 68-967.1, and UCDFST 74-1030 were isolated in USA, respectively, from cottonwood tree Populus deltoides in 1971 (Davis, CA), slime flux of Quercus sp. in 1954 (CA), exudate of black oak Q. kelloggii in 1954 (Central Sierra Nevada. CA), dry frass from Salix sp. in 1968 (Soleduck Road, Olympic National Park, WA) and from flux of hackberry tree Celtis sp. in 1974 (CA). The new species was differentiated from Komagataella kurtzmanii, Komagataella pastoris, Komagataella phaffii, Komagataella populi, Komagataella pseudopastoris and Komagataella ulmi by divergence in gene sequences for D1/D2 LSU rRNA, ITS1-5.8S-ITS2, RNA polymerase subunit I and translation elongation factor-1α. Komagataella mondaviorum sp. nov. is registered in MycoBank under MB 821789.

摘要

从美国加利福尼亚大学戴维斯分校的法夫酵母菌种保藏中心获得的5株甲基营养型菌株(UCDFST 71-1024、UCDFST 54-11.16、UCDFST 54-11.141、UCDFST 68-967.1和UCDFST 74-1030),最初被鉴定为巴斯德毕赤酵母,现发现它们代表一个新的科马加塔酵母属物种。蒙氏科马加塔酵母新种菌株UCDFST 71-(模式菌株)=CBS 15017、UCDFST 54-11.16、UCDFST 54-11.141、UCDFST 68-967.1和UCDFST 74-1030分别于1971年在美国加利福尼亚州戴维斯从三角叶杨中分离得到,1954年在加利福尼亚州从栎属树木的流胶中分离得到,1954年在加利福尼亚州内华达山脉中部从凯洛格栎的渗出物中分离得到,1968年在华盛顿州奥林匹克国家公园索莱达克路从柳属植物的干粪便中分离得到,1974年在加利福尼亚州从朴属树木的流胶中分离得到。通过D1/D2 LSU rRNA、ITS1-5.8S-ITS2、RNA聚合酶亚基I和翻译延伸因子-1α的基因序列差异,将该新物种与库氏科马加塔酵母、巴斯德科马加塔酵母、法夫科马加塔酵母、杨科马加塔酵母、假巴斯德科马加塔酵母和榆科马加塔酵母区分开来。蒙氏科马加塔酵母新种在MycoBank中的登记号为MB 821789。

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