Institute of Materials Research, Helmholtz-Zentrum Geesthacht (HZG), Geesthacht, Germany,
J Mater Sci Mater Med. 2013 Oct;24(10):2337-58. doi: 10.1007/s10856-013-4989-z. Epub 2013 Jun 27.
Despite its non-matching mechanical properties titanium remains the preferred metal implant material in orthopaedics. As a consequence in some cases stress shielding effect occurs, leading to implant loosening, osteopenia, and finally revision surgery. Porous metal scaffolds to allow easier specialised cells ingrowth with mechanical properties closer to the ones of bone can overcome this problem. This should improve healing processes, implant integration, and dynamic strength of implants retaining. Three Ti-6Al-4V materials were metal injection moulded and tailored porosities were effectively achieved. After microstructural and mechanical characterisation, two different primary cells of mesenchymal origin (human umbilical cord perivascular cells and human bone derived cells which revealed to be two pertinent models) as well as one cell line originated from primary osteogenic sarcoma, Saos-2, were bestowed to investigate cell-material interaction on genomic and proteome levels. Biological examinations disclosed that no material has negative impact on early adhesion, proliferation or cell viability. An efficient cell ingrowth into material with an average porosity of 25-50 μm was proved.
尽管钛的机械性能不匹配，但它仍然是骨科中首选的金属植入材料。因此，在某些情况下会出现应力屏蔽效应，导致植入物松动、骨质疏松，最终需要进行翻修手术。具有更接近骨骼机械性能的多孔金属支架可以促进专门细胞的更容易生长，从而克服这个问题。这将改善愈合过程、植入物的整合以及保留植入物的动态强度。三种 Ti-6Al-4V 材料采用金属注射成型技术进行成型，并有效地实现了定制的多孔性。在进行微观结构和机械特性表征后，选择了两种不同的间充质来源的原代细胞（人脐带血管周围细胞和人骨源细胞，这两种细胞被证明是两种相关的模型）以及一种源自原发性成骨肉瘤的细胞系 Saos-2，用于在基因组和蛋白质组水平上研究细胞-材料相互作用。生物学研究表明，没有一种材料对早期黏附、增殖或细胞活力有负面影响。证明了具有 25-50μm 平均孔隙率的材料中能够有效地促进细胞生长。
