de Paiva Rolla Amanda Alves, de Fátima Corrêa Carvalho Josirley, Fuganti-Pagliarini Renata, Engels Cibelle, do Rio Alexandre, Marin Silvana Regina Rockenbach, de Oliveira Maria Cristina Neves, Beneventi Magda A, Marcelino-Guimarães Francismar Corrêa, Farias José Renato Bouças, Neumaier Norman, Nakashima Kazuo, Yamaguchi-Shinozaki Kazuko, Nepomuceno Alexandre Lima
Department of General Biology, Londrina State University, Rodovia Celso Garcia Cid, PR 445, Km 380, P.O. Box 6001, Londrina, 86051-990, Brazil,
Transgenic Res. 2014 Feb;23(1):75-87. doi: 10.1007/s11248-013-9723-6. Epub 2013 Jun 27.
The development of drought tolerant plants is a high priority because the area suffering from drought is expected to increase in the future due to global warming. One strategy for the development of drought tolerance is to genetically engineer plants with transcription factors (TFs) that regulate the expression of several genes related to abiotic stress defense responses. This work assessed the performance of soybean plants overexpressing the TF DREB1A under drought conditions in the field and in the greenhouse. Drought was simulated in the greenhouse by progressively drying the soil of pot cultures of the P58 and P1142 lines. In the field, the performance of the P58 line and of 09D-0077, a cross between the cultivars BR16 and P58, was evaluated under four different water regimes: irrigation, natural drought (no irrigation) and water stress created using rain-out shelters in the vegetative or reproductive stages. Although the dehydration-responsive element-binding protein (DREB) plants did not outperform the cultivar BR16 in terms of yield, some yield components were increased when drought was introduced during the vegetative stage, such as the number of seeds, the number of pods with seeds and the total number of pods. The greenhouse data suggest that the higher survival rates of DREB plants are because of lower water use due to lower transpiration rates under well watered conditions. Further studies are needed to better characterize the soil and atmospheric conditions under which these plants may outperform the non-transformed parental plants.
培育耐旱植物是当务之急，因为由于全球变暖，预计未来干旱受灾面积将会增加。培育耐旱性的一种策略是通过基因工程使植物表达转录因子（TFs），这些转录因子可调控多个与非生物胁迫防御反应相关的基因。这项研究评估了在田间和温室干旱条件下过表达TF DREB1A的大豆植株的表现。在温室中，通过逐渐干燥P58和P1142品系盆栽土壤来模拟干旱。在田间，在四种不同水分条件下评估了P58品系以及09D - 0077（BR16和P58品种的杂交种）的表现：灌溉、自然干旱（不灌溉）以及在营养期或生殖期使用防雨棚造成的水分胁迫。尽管在产量方面，脱水响应元件结合蛋白（DREB）植物并未超过BR16品种，但在营养期引入干旱时，一些产量构成因素有所增加，例如种子数量、有种子的豆荚数量和豆荚总数。温室数据表明，DREB植物较高的存活率是因为在水分充足的条件下蒸腾速率较低，从而水分利用较少。需要进一步研究以更好地描述这些植物可能优于未转化亲本植物的土壤和大气条件。
