State Key Lab of Agrobiotechnology, National Animal Transmissible Spongiform Encephalopathy Laboratory, College of Veterinary Medicine, China Agricultural University, Beijing, China.
DNA Cell Biol. 2013 Aug;32(8):480-6. doi: 10.1089/dna.2012.1941. Epub 2013 Jun 29.
Mycobacterium bovis, the classical causative agent of bovine tuberculosis (BTB), infects animals of agricultural importance and other mammals, including humans. Neutrophils are one of the first lines of defense against all microbes and produce a diverse collection of antimicrobial molecules, which play an important role in the early control of tuberculosis progression. An interferon (IFN)-inducible neutrophil-driven blood transcriptional signature that consisted of both IFN-γ and type I IFN-α/β signaling has been identified in human tuberculosis, supporting a role for neutrophils in the pathogenesis of tuberculosis disease. However, it is unknown whether bovine neutrophils play a similar role during M. bovis infection. Thus, we assessed the expression levels of ten IFN-inducible transcriptional genes in neutrophils from healthy cattle stimulated by M. bovis and neutrophils isolated from three groups of cattle of different infection status, and in addition, examined the changes in the expression of myeloperoxidase (MPO) and pentraxin-related protein pentraxin-inducible protein (PTX3) genes during bovine tuberculosis infection. Our results demonstrated a specific expression pattern of IFN-inducible transcriptional genes and MPO and PTX3 genes in neutrophils during bovine tuberculosis infection. The observed expression pattern provides a potential diagnostic tool, which may have implications for vaccine and therapeutic development to combat the bovine tuberculosis epidemic.
牛分枝杆菌是牛结核病（BTB）的经典病原体，感染农业重要动物和其他哺乳动物，包括人类。中性粒细胞是抵御所有微生物的第一道防线之一，产生多种抗菌分子，在早期控制结核病进展中发挥重要作用。在人类结核病中已经确定了由 IFN-γ 和 I 型 IFN-α/β信号组成的干扰素（IFN）诱导的中性粒细胞驱动的血液转录特征，支持中性粒细胞在结核病发病机制中的作用。然而，尚不清楚牛中性粒细胞在 M. bovis 感染期间是否发挥类似作用。因此，我们评估了由 M. bovis 刺激的健康牛中性粒细胞和来自不同感染状态的三组牛中性粒细胞中十个 IFN 诱导的转录基因的表达水平，并检查了在牛结核病感染期间髓过氧化物酶（MPO）和五聚蛋白相关蛋白五聚蛋白诱导蛋白（PTX3）基因表达的变化。我们的结果表明，在牛结核病感染期间，中性粒细胞中 IFN 诱导的转录基因以及 MPO 和 PTX3 基因的表达具有特定的模式。观察到的表达模式提供了一种潜在的诊断工具，这可能对疫苗和治疗性开发以对抗牛结核病流行具有重要意义。
