Kano R, Nakamura Y, Watari T, Watanabe S, Takahashi H, Tsujimoto H, Hasegawa A
Department of Veterinary Internal Medicine, Graduate School of Agricultural and Life Sciences, University of Tokyo, Japan.
Mycoses. 1998 Mar-Apr;41(3-4):139-43. doi: 10.1111/j.1439-0507.1998.tb00315.x.
Clinical isolates of Microsporum canis and M. gypseum from humans, dogs and cats were examined by random amplification of polymorphic DNA (RAPD) and Southern hybridization analyses. The RAPD band patterns of six clinical isolates of M. canis were identical to those of standard strains of Arthroderma otae. Of nine clinical isolates of M. gypseum seven and two isolates showed RAPD patterns identical to those of standard strains of A. gypseum and A. incurvatum respectively. Southern blot analysis using a probe (C3) obtained from A. otae DNA revealed that six clinical isolates of M. canis showed specific bands identical to those detected in the standard strains of A. otae. Of nine clinical isolates of M. gypseum, seven and two isolates showed bands hybridized by the C3 probe identical to those detected in A. gypseum and A. incurvatum respectively. Furthermore, the results from mating experiments on these nine clinical isolates of M. gypseum showed complete agreement with the results from RAPD and Southern hybridization analyses. These findings clearly indicate that RAPD and Southern hybridization analyses are very useful in the identification of clinical isolates of M. canis and M. gypseum.
采用随机扩增多态性DNA（RAPD）和Southern杂交分析方法，对来自人类、犬类和猫类的犬小孢子菌和石膏样小孢子菌临床分离株进行了检测。6株犬小孢子菌临床分离株的RAPD条带模式与奥塔节皮菌标准菌株的条带模式相同。在9株石膏样小孢子菌临床分离株中，7株和2株的RAPD模式分别与石膏样节皮菌和弯曲节皮菌标准菌株的RAPD模式相同。使用从奥塔节皮菌DNA获得的探针（C3）进行Southern印迹分析表明，6株犬小孢子菌临床分离株显示出与奥塔节皮菌标准菌株中检测到的特异性条带相同。在9株石膏样小孢子菌临床分离株中，7株和2株显示出与C3探针杂交的条带，分别与在石膏样节皮菌和弯曲节皮菌中检测到的条带相同。此外，对这9株石膏样小孢子菌临床分离株进行的交配实验结果与RAPD和Southern杂交分析结果完全一致。这些发现清楚地表明，RAPD和Southern杂交分析在犬小孢子菌和石膏样小孢子菌临床分离株的鉴定中非常有用。
