与后肢固定大鼠的成骨细胞相比，脊髓损伤大鼠分离出的成骨细胞对机械负荷的反应较小。
Isolated osteoblasts from spinal cord-injured rats respond less to mechanical loading as compared with those from hindlimb-immobilized rats.
作者信息
Jiang Sheng-Dan, Yang Yue-Hua, Chen Jiang-Wei, Jiang Lei-Sheng
机构信息
Department of Orthopaedic Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.
出版信息
J Spinal Cord Med. 2013 May;36(3):220-4. doi: 10.1179/2045772312Y.0000000071.
BACKGROUND
The pathogenesis of osteoporosis after spinal cord injury (SCI) may be different from disuse osteoporosis.
OBJECTIVE
To investigate whether there is the differential anabolic response to mechanical loading between osteoblasts from SCI rats and those from hindlimb-immobilized rats.
METHODS
Femoral bone-marrow was harvested for osteoblast culture from SCI rats, hindlimb-immobilized rats, and control rats 3 weeks after animal model creation. At the stage of differentiation, rat osteoblasts were plated in six-well plates for stretching. Cyclic strains were applied for 48 hours, and then alkaline phosphatase (ALPase) activity, procollagen, and osteocalcin production, and gene expression of osteocalcin, runt-related transcription factor 2 (Runx2), and osterix were measured in osteoblasts from SCI rats, hindlimb-immobilized rats, and control rats.
RESULTS
ALPase activity, procollagen, and osteocalcin production, and gene expression of osteocalcin, Runx2, and osterix were significantly lower in osteoblasts after stretching from SCI rats compared with those from hindlimb-immobilized rats. However, there was no significant difference of these parameters between isolated osteoblasts from hindlimb-immobilized rats and those from control rats.
CONCLUSION
The activity of isolated osteoblasts from SCI rats was lower than control rats, and this suggested that osteoblasts from SCI rats responded less to mechanical loading as compared with those from control rats.
背景
脊髓损伤（SCI）后骨质疏松的发病机制可能与废用性骨质疏松不同。
目的
探讨SCI大鼠成骨细胞与后肢固定大鼠成骨细胞对机械负荷的合成代谢反应是否存在差异。
方法
在动物模型建立3周后，从SCI大鼠、后肢固定大鼠和对照大鼠中采集股骨骨髓进行成骨细胞培养。在分化阶段，将大鼠成骨细胞接种于六孔板中进行拉伸。施加循环应变48小时，然后检测SCI大鼠、后肢固定大鼠和对照大鼠成骨细胞中的碱性磷酸酶（ALPase）活性、前胶原和骨钙素生成，以及骨钙素、 runt相关转录因子2（Runx2）和osterix的基因表达。
结果
与后肢固定大鼠相比，SCI大鼠拉伸后成骨细胞中的ALPase活性、前胶原和骨钙素生成，以及骨钙素、Runx2和osterix的基因表达显著降低。然而，后肢固定大鼠分离的成骨细胞与对照大鼠分离的成骨细胞之间这些参数无显著差异。
结论
SCI大鼠分离的成骨细胞活性低于对照大鼠，这表明与对照大鼠相比，SCI大鼠的成骨细胞对机械负荷的反应较小。
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