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Effects of spinal cord injury and hindlimb immobilization on sublesional and supralesional bones in young growing rats.脊髓损伤和后肢固定对幼年生长大鼠损伤节段以下及以上骨骼的影响。
Bone. 2008 Jul;43(1):119-125. doi: 10.1016/j.bone.2008.03.015. Epub 2008 Apr 8.
2
Mechanical stress-mediated Runx2 activation is dependent on Ras/ERK1/2 MAPK signaling in osteoblasts.机械应力介导的Runx2激活依赖于成骨细胞中的Ras/ERK1/2丝裂原活化蛋白激酶信号通路。
J Cell Biochem. 2007 Aug 1;101(5):1266-77. doi: 10.1002/jcb.21249.
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Mechanisms of osteoporosis in spinal cord injury.脊髓损伤中骨质疏松的机制。
Clin Endocrinol (Oxf). 2006 Nov;65(5):555-65. doi: 10.1111/j.1365-2265.2006.02683.x.
4
Effects of spinal cord injury on osteoblastogenesis, osteoclastogenesis and gene expression profiling in osteoblasts in young rats.脊髓损伤对幼鼠成骨细胞生成、破骨细胞生成及成骨细胞基因表达谱的影响
Osteoporos Int. 2007 Mar;18(3):339-49. doi: 10.1007/s00198-006-0229-4. Epub 2006 Oct 12.
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Modulation of ERK 1/2 and p38 MAPK signaling pathways by ATP in osteoblasts: involvement of mechanical stress-activated calcium influx, PKC and Src activation.ATP对成骨细胞中ERK 1/2和p38 MAPK信号通路的调节作用：机械应力激活的钙内流、蛋白激酶C（PKC）及Src激活的参与
Int J Biochem Cell Biol. 2006;38(12):2082-91. doi: 10.1016/j.biocel.2006.05.018. Epub 2006 Jul 1.
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Osteoporosis after spinal cord injury.脊髓损伤后的骨质疏松症。
Osteoporos Int. 2006 Feb;17(2):180-92. doi: 10.1007/s00198-005-2028-8. Epub 2005 Oct 11.
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Mechanical control of human osteoblast apoptosis and proliferation in relation to differentiation.与分化相关的人成骨细胞凋亡和增殖的机械控制
Calcif Tissue Int. 2003 Apr;72(4):505-12. doi: 10.1007/s00223-002-2027-0. Epub 2003 Jan 21.
8
Intensive exercise may preserve bone mass of the upper limbs in spinal cord injured males but does not retard demineralisation of the lower body.高强度运动可能会保留脊髓损伤男性上肢的骨量，但不会延缓下半身的骨质流失。
Spinal Cord. 2002 May;40(5):230-5. doi: 10.1038/sj.sc.3101286.
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Disuse osteoporosis.废用性骨质疏松
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Bone mineral and geometric changes through the femur with immobilization due to spinal cord injury.脊髓损伤导致制动时股骨的骨矿物质及几何形态变化。
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与后肢固定大鼠的成骨细胞相比，脊髓损伤大鼠分离出的成骨细胞对机械负荷的反应较小。

Isolated osteoblasts from spinal cord-injured rats respond less to mechanical loading as compared with those from hindlimb-immobilized rats.

作者信息

Jiang Sheng-Dan, Yang Yue-Hua, Chen Jiang-Wei, Jiang Lei-Sheng

机构信息

Department of Orthopaedic Surgery, Xinhua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, China.

出版信息

J Spinal Cord Med. 2013 May;36(3):220-4. doi: 10.1179/2045772312Y.0000000071.

DOI:10.1179/2045772312Y.0000000071
PMID:23809592
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3654448/
Abstract

BACKGROUND

The pathogenesis of osteoporosis after spinal cord injury (SCI) may be different from disuse osteoporosis.

OBJECTIVE

To investigate whether there is the differential anabolic response to mechanical loading between osteoblasts from SCI rats and those from hindlimb-immobilized rats.

METHODS

Femoral bone-marrow was harvested for osteoblast culture from SCI rats, hindlimb-immobilized rats, and control rats 3 weeks after animal model creation. At the stage of differentiation, rat osteoblasts were plated in six-well plates for stretching. Cyclic strains were applied for 48 hours, and then alkaline phosphatase (ALPase) activity, procollagen, and osteocalcin production, and gene expression of osteocalcin, runt-related transcription factor 2 (Runx2), and osterix were measured in osteoblasts from SCI rats, hindlimb-immobilized rats, and control rats.

RESULTS

ALPase activity, procollagen, and osteocalcin production, and gene expression of osteocalcin, Runx2, and osterix were significantly lower in osteoblasts after stretching from SCI rats compared with those from hindlimb-immobilized rats. However, there was no significant difference of these parameters between isolated osteoblasts from hindlimb-immobilized rats and those from control rats.

CONCLUSION

The activity of isolated osteoblasts from SCI rats was lower than control rats, and this suggested that osteoblasts from SCI rats responded less to mechanical loading as compared with those from control rats.

摘要

背景

脊髓损伤（SCI）后骨质疏松的发病机制可能与废用性骨质疏松不同。

目的

探讨SCI大鼠成骨细胞与后肢固定大鼠成骨细胞对机械负荷的合成代谢反应是否存在差异。

方法

在动物模型建立3周后，从SCI大鼠、后肢固定大鼠和对照大鼠中采集股骨骨髓进行成骨细胞培养。在分化阶段，将大鼠成骨细胞接种于六孔板中进行拉伸。施加循环应变48小时，然后检测SCI大鼠、后肢固定大鼠和对照大鼠成骨细胞中的碱性磷酸酶（ALPase）活性、前胶原和骨钙素生成，以及骨钙素、 runt相关转录因子2（Runx2）和osterix的基因表达。

结果

与后肢固定大鼠相比，SCI大鼠拉伸后成骨细胞中的ALPase活性、前胶原和骨钙素生成，以及骨钙素、Runx2和osterix的基因表达显著降低。然而，后肢固定大鼠分离的成骨细胞与对照大鼠分离的成骨细胞之间这些参数无显著差异。

结论

SCI大鼠分离的成骨细胞活性低于对照大鼠，这表明与对照大鼠相比，SCI大鼠的成骨细胞对机械负荷的反应较小。