Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
J Chromatogr A. 2013 Aug 2;1301:237-42. doi: 10.1016/j.chroma.2013.05.057. Epub 2013 Jun 1.
We developed a practical analytical system for high-throughput and comprehensive lipid profiling using a supercritical fluid chromatography (SFC) system coupled to an Orbitrap Fourier transform mass spectrometer (Orbitrap FT-MS). Using our SFC method, polar lipid molecular species were separated based on not only their fatty acyl moieties but also their polar head groups, using a single octadecylsilyl (ODS) column. In addition, because automatic data processing software was used for the identification of lipid molecular species, the analysis time including data processing was about a half an hour per sample. A variety of lipid molecular species were detected in mouse plasma, and isomers which often co-elute in reverse phase separation were identified accurately and quantified individually. To the best of our knowledge, this is the first report describing the chromatographic separation of lipids based on both fatty acyl moieties and polar head groups, using a single ODS column. Our results demonstrate that SFC/MS is a powerful tool for the simultaneous analysis of diverse lipid molecular species.
我们开发了一种实用的分析系统，用于使用超临界流体色谱（SFC）系统与轨道阱傅里叶变换质谱仪（Orbitrap FT-MS）联用进行高通量和全面的脂质分析。使用我们的 SFC 方法，基于单一的十八烷基硅烷（ODS）柱，不仅可以根据其脂肪酸部分，还可以根据其极性头部基团分离极性脂质分子种类。此外，由于用于鉴定脂质分子种类的自动数据处理软件的使用，包括数据处理在内的每个样品的分析时间约为半小时。在小鼠血浆中检测到多种脂质分子种类，并且能够准确地鉴定和单独定量通常在反相分离中共同洗脱的异构体。据我们所知，这是第一个描述使用单一 ODS 柱根据脂肪酸部分和极性头部基团进行脂质色谱分离的报告。我们的结果表明，SFC/MS 是同时分析多种脂质分子种类的有力工具。
