Addepalli Ramani Venkata, Mullangi Ramesh
Palm Meadows, Kompally, Hyderabad-500100, India.
Laxai Life Sciences Pvt Ltd, MN Park, Genome Valley, Shamirpet, Hyderabad-500 078, India.
ADMET DMPK. 2020 Dec 9;9(1):1-22. doi: 10.5599/admet.913. eCollection 2021.
Lipids are a complex and critical heterogeneous molecular entity, playing an intricate and key role in understanding biological activities and disease processes. Lipidomics aims to quantitatively define the lipid classes, including their molecular species. The analysis of the biological tissues and fluids are challenging due to the extreme sample complexity and occurrence of the molecular species as isomers or isobars. This review documents the overview of lipidomics workflow, beginning from the approaches of sample preparation, various analytical techniques and emphasizing the state-of-the-art mass spectrometry either by shotgun or coupled with liquid chromatography. We have considered the latest ion mobility spectroscopy technologies to deal with the vast number of structural isomers, different imaging techniques. All these techniques have their pitfalls and we have discussed how to circumvent them after reviewing the power of each technique with examples..
脂质是一种复杂且关键的异质分子实体，在理解生物活性和疾病过程中发挥着复杂而关键的作用。脂质组学旨在定量定义脂质类别，包括其分子种类。由于生物组织和流体样本的极端复杂性以及分子种类以异构体或同量异位素形式存在，对其进行分析具有挑战性。本综述记录了脂质组学工作流程的概述，从样品制备方法、各种分析技术开始，并着重介绍了通过鸟枪法或与液相色谱联用的最新质谱技术。我们还考虑了用于处理大量结构异构体的最新离子迁移谱技术以及不同的成像技术。所有这些技术都有其缺陷，我们在通过实例回顾每种技术的优势后讨论了如何规避这些缺陷。
