Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, A-8010 Graz, Austria.
Trends Biotechnol. 2013 Aug;31(8):468-78. doi: 10.1016/j.tibtech.2013.05.005. Epub 2013 Jun 25.
Owing to the more abundant occurrence of racemic compounds compared to prochiral or meso forms, most enantiomerically pure products are obtained via racemate resolution. This review summarizes (chemo)enzymatic enantioconvergent processes based on the use of hydrolytic enzymes, which are able to invert a stereocenter during catalysis that can overcome the 50%-yield limitation of kinetic resolution. Recent developments are presented in the fields of inverting or retaining sulfatases, epoxide hydrolases and dehalogenases, which allow the production of secondary alcohols or vicinal diols at a 100% theoretical yield from a racemate via enantioconvergent processes.
由于外消旋化合物比前手性或内消旋形式更丰富，因此大多数对映体纯的产物都是通过外消旋拆分得到的。本综述总结了基于使用水解酶的（化学）酶促对映体转化过程，这些酶能够在催化过程中反转一个立体中心，从而克服动力学拆分的 50%产率限制。介绍了反转或保留硫酸酯酶、环氧化物水解酶和脱卤酶领域的最新进展，这些酶可以通过对映体转化过程从外消旋混合物中以 100%理论产率生产仲醇或顺二醇。
