Zhang Wei-Wei, Jia Jun-Qi, Wang Na, Hu Cheng-Li, Yang Sheng-Yong, Yu Xiao-Qi
Key Laboratory of Green Chemistry & Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, PR China.
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu 610064, PR China.
Biotechnol Rep (Amst). 2015 Apr 16;7:1-8. doi: 10.1016/j.btre.2015.04.003. eCollection 2015 Sep.
Microemulsion-based organogels (MBGs) were effectively employed for the immobilization of four commonly used lipases. During the asymmetric hydrolysis of ketoprofen vinyl ester at 30 °C for 24 h, lipase from i and immobilized in microemulsion-based organogels (RML MBGs and MJL MBGs) maintained good enantioselectivities ( were 86.2% and 99.2%, respectively), and their activities increased 12.8-fold and 7.8-fold, respectively, compared with their free forms. They gave higher yields compared with other lipase MBGs and exhibited better enantioselectivity than commercial immobilized lipases. Immobilization considerably increased the tolerance to organic solvents and high temperature. Both MJL MBGs and RML MBGs showed excellent reusability during 30 cycles of repeated 24 h reactions at 30 °C (over 40 days). The system maintained yields of greater than 50%, while the values of RML MBGs and MJL MBGs remained nearly constant at 95% and 88%, respectively.
基于微乳液的有机凝胶（MBGs）被有效地用于固定四种常用的脂肪酶。在30℃下对酮洛芬乙烯酯进行24小时的不对称水解过程中，固定在基于微乳液的有机凝胶（RML MBGs和MJL MBGs）中的脂肪酶i和保持了良好的对映选择性（分别为86.2%和99.2%），并且与它们的游离形式相比，它们的活性分别提高了12.8倍和7.8倍。与其他脂肪酶MBGs相比，它们的产率更高，并且比商业固定化脂肪酶表现出更好的对映选择性。固定化显著提高了对有机溶剂和高温的耐受性。在30℃下进行30个循环的24小时重复反应（超过40天）期间，MJL MBGs和RML MBGs都表现出优异的可重复使用性。该系统保持了大于50%的产率，而RML MBGs和MJL MBGs的对映体过量值分别在95%和88%左右保持几乎恒定。
