Portland Alcohol Research Center, Veterans Affairs Medical Center, Oregon Health & Science University, Portland, OR 97239, USA.
Alcohol. 2013 Aug;47(5):381-9. doi: 10.1016/j.alcohol.2013.05.002.
Withdrawal Seizure-Prone (WSP) and Withdrawal Seizure-Resistant (WSR) mouse lines were bidirectionally selectively bred, respectively, to have severe or mild ethanol withdrawal handling-induced convulsions (HICs) after cessation of 3 days of ethanol vapor inhalation. Murine genotypes with severe withdrawal have been found to show low ethanol consumption, and high consumers show low withdrawal. An early drinking study with WSP and WSR mice showed modest evidence consistent with this genetic correlation, but there were several limitations to that experiment. We therefore conducted a thorough assessment of two bottle ethanol preference drinking in both replicate pairs of WSP/WSR selected lines in mice of both sexes. Greater preference drinking of WSR-2 than WSP-2 female mice confirmed the earlier report. However, in the parallel set of selected lines, the WSP-1 mice drank more than the WSR-1s. Naive mice tested for preference for sucrose, saccharin and quinine did not differ markedly for any tastant. Finally, in a test of binge-like drinking, Drinking in the Dark (DID), WSP mice drank more than WSR mice and attained significantly higher (but still modest) blood ethanol concentrations. Tests of acute withdrawal after DID showed a mild, but significant elevation in handling-induced convulsions in the WSP line. These results provide further evidence that 2-bottle ethanol preference and DID are genetically distinguishable traits.
撤药发作易感性（WSP）和撤药发作抗性（WSR）小鼠系分别通过有方向的选择性繁殖，产生严重或轻度乙醇撤药处理诱导的抽搐（HIC），在停止 3 天的乙醇蒸汽吸入后。具有严重撤药的小鼠基因型已被发现表现出低乙醇消耗，而高消费者表现出低撤药。WSP 和 WSR 小鼠的早期饮酒研究提供了与这种遗传相关性一致的适度证据，但该实验存在几个限制。因此，我们在雄性和雌性 WSP/WSR 选择系的两个重复对中进行了彻底评估，评估了它们在两瓶乙醇偏好性饮用中的情况。WSR-2 雌鼠比 WSP-2 雌鼠表现出更大的偏好性饮酒，证实了之前的报道。然而，在平行的选择系中，WSP-1 小鼠比 WSR-1 小鼠喝得更多。用于测试蔗糖、糖精和奎宁偏好性的新生小鼠在任何味觉上都没有明显差异。最后，在 binge-like 饮酒测试——暗室饮酒（DID）中，WSP 小鼠比 WSR 小鼠喝得更多，并且达到了明显更高（但仍适度）的血液乙醇浓度。DID 后急性撤药测试显示 WSP 系处理诱导的抽搐有轻微但显著升高。这些结果进一步证明了两瓶乙醇偏好性和 DID 是遗传上可区分的特征。
