Palmieri C, Riccardi E
School of Veterinary Science, University of Queensland, Gatton Campus, Gatton 4343, Queensland, Australia.
J Comp Pathol. 2013 Nov;149(4):417-23. doi: 10.1016/j.jcpa.2013.05.001. Epub 2013 Jun 25.
Homeobox genes are known to be examples of the intimate relationship between embryogenesis and tumourigenesis. Specifically, the HOXA13 gene plays a fundamental role in the development of the urogenital tract and external genitalia and in prostate organogenesis. There are no reports on the expression of HOXA13 in normal, hyperplastic or neoplastic canine prostate tissue or in other types of tumours. Six normal, 16 hyperplastic and 12 neoplastic canine prostates were examined microscopically and immunohistochemically with a polyclonal antibody specific for human HOXA13. An immunohistochemical score was generated. HOXA13 was expressed in the cytoplasm of epithelial cells in normal, hyperplastic and neoplastic prostates. The percentage of immunolabelled cells in all prostatic carcinomas (PCs) was greatly increased, with a score of 85.3 (±5.25) compared with normal (2 ± 0.71) and hyperplastic prostates (6.08 ± 2.21). The increase in HOXA13 expression in canine PCs suggests the involvement of this transcription factor in carcinogenesis and promotion of tumour growth.
已知同源盒基因是胚胎发生与肿瘤发生之间密切关系的实例。具体而言，HOXA13基因在泌尿生殖道和外生殖器的发育以及前列腺器官发生中起着重要作用。目前尚无关于HOXA13在正常、增生或肿瘤性犬前列腺组织或其他类型肿瘤中表达的报道。使用针对人HOXA13的多克隆抗体对6个正常、16个增生和12个肿瘤性犬前列腺进行了显微镜检查和免疫组织化学检查。生成了免疫组织化学评分。HOXA13在正常、增生和肿瘤性前列腺的上皮细胞胞质中表达。与正常前列腺（2±0.71）和增生性前列腺（6.08±2.21）相比，所有前列腺癌（PC）中免疫标记细胞的百分比大幅增加，评分为85.3（±5.25）。犬PC中HOXA13表达的增加表明该转录因子参与了致癌作用并促进肿瘤生长。
