Palmieri C, Mancini M, Benazzi C, Della Salda L
School of Veterinary Science, University of Queensland, Gatton Campus, Gatton 4343, Queensland, Australia.
Faculty of Veterinary Medicine, Teramo University, Piazza Aldo Moro 45, 64100 Teramo, Italy.
J Comp Pathol. 2014 May;150(4):393-8. doi: 10.1016/j.jcpa.2014.01.006. Epub 2014 Jan 28.
Heat shock protein 90 (HSP90) is a molecular chaperone that regulates critical signalling proteins of cancer development and progression. Abnormal levels of HSP90 have been observed in human prostatic carcinoma (PC), with prognostic and therapeutic implications. Since spontaneously arising canine PC is a valuable model for the human disease, the aim of this study was to evaluate the immunohistochemical expression of HSP90 in two normal canine prostates, 17 canine prostates with benign prostatic hyperplasia (BPH) and five canine prostates with PC. HSP90 was expressed in the cytoplasm of epithelial cells in all samples, with a significant increase in labelled cells in PCs. Nuclear labelling was observed occasionally in normal tissue, but was increased in BPH and PC. HSP90 immunoreactivity in preneoplastic lesions (proliferative inflammatory atrophy and prostatic intraepithelial neoplasia) was similar to that in PCs. Increased HSP90 expression in canine PCs suggests the involvement of this molecule in carcinogenesis and tumour progression, supporting HSP90 as a potential target for therapeutic intervention.
热休克蛋白90（HSP90）是一种分子伴侣，可调节癌症发生和发展的关键信号蛋白。在人类前列腺癌（PC）中已观察到HSP90水平异常，具有预后和治疗意义。由于自发产生的犬类PC是人类疾病的宝贵模型，本研究的目的是评估HSP90在两个正常犬前列腺、17个患有良性前列腺增生（BPH）的犬前列腺和5个患有PC的犬前列腺中的免疫组织化学表达。HSP90在所有样本的上皮细胞胞质中表达，PC中标记细胞显著增加。在正常组织中偶尔观察到核标记，但在BPH和PC中增加。癌前病变（增殖性炎性萎缩和前列腺上皮内瘤变）中的HSP90免疫反应性与PC中的相似。犬类PC中HSP90表达增加表明该分子参与致癌作用和肿瘤进展，支持HSP90作为治疗干预的潜在靶点。
