Tianjin Key Laboratory of Animal and Plant Resistance, College of Life Science, Tianjin Normal University, Tianjin 300387, PR China.
Fish Shellfish Immunol. 2013 Sep;35(3):840-6. doi: 10.1016/j.fsi.2013.06.018. Epub 2013 Jun 25.
Peritrophin was first separated from insect peritrophic membrane (PM), and it played an important role in stimulating the digestion of food and protecting insects from invasion by microorganisms. In this study, a full-length cDNA of a new peritrophin-like protein (EcPT) was cloned from the ridgetail white shrimp Exopalaemon carinicauda, which was an excellent experimental animal for shrimp. The full length cDNA comprised 1235 bp including an 873 bp open reading frame encoding 291 amino acids. The deduced amino acid sequence contained a segment of signal peptide and three conserved chitin binding type 2 domains (ChtBD2) characterized by having a 6-cysteine motif. Tissue expression analysis revealed that EcPT was mainly expressed in stomach and gills, which were also the two main target tissues of WSSV infection. The transcription levels of EcPT in both stomach and gills were found to have significantly changed upon WSSV infection by real-time PCR. Silencing EcPT by dsRNA interference led to higher survival rate of shrimp against WSSV challenge, which suggested that EcPT might be involved in WSSV infection.
围食膜蛋白最初是从昆虫围食膜中分离出来的，它在刺激食物消化和保护昆虫免受微生物入侵方面发挥着重要作用。在本研究中，从脊尾白虾（Exopalaemon carinicauda）中克隆了一种新的围食膜蛋白样蛋白（EcPT）的全长 cDNA，脊尾白虾是虾类的一种优秀实验动物。全长 cDNA 包含 1235bp，其中包括一个编码 291 个氨基酸的 873bp 开放阅读框。推导的氨基酸序列包含一段信号肽和三个保守的几丁质结合型 2 结构域（ChtBD2），其特征是具有 6 个半胱氨酸基序。组织表达分析显示，EcPT 主要在胃和鳃中表达，这也是 WSSV 感染的两个主要靶组织。实时 PCR 分析发现，EcPT 在胃和鳃中的转录水平在 WSSV 感染后均发生了显著变化。通过 dsRNA 干扰沉默 EcPT 导致虾对 WSSV 攻击的存活率更高，这表明 EcPT 可能参与了 WSSV 感染。
