Mohd Ghani Farhana, Bhassu Subha
Department of Genetics & Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
Centre for Research in Biotechnology for Agriculture (CEBAR), University of Malaya, Kuala Lumpur, Malaysia.
PeerJ. 2019 Dec 20;7:e8107. doi: 10.7717/peerj.8107. eCollection 2019.
The emergence of diseases such as white spot disease has become a threat to cultivation. Although there have been a few studies utilizing RNA-Seq, the cellular processes of host-virus interaction in this species remain mostly anonymous. In the present study, was challenged with WSSV by intramuscular injection and survived for 12 days. The effect of the host gene expression by WSSV infection in the haemocytes, hepatopancreas and muscle of was studied using Illumina HiSeq 2000. The RNA-Seq of cDNA libraries was developed from surviving WSSV-challenged shrimp as well as from normal healthy shrimp as control. A comparison of the transcriptome data of the two groups showed 2,644 host genes to be significantly up-regulated and 2,194 genes significantly down-regulated as a result of the infection with WSSV. Among the differentially expressed genes, our study discovered HMGB, TNFSF and c-Jun in as new potential candidate genes for further investigation for the development of potential disease resistance markers. Our study also provided significant data on the differential expression of genes in the survived WSSV infected that will help to improve understanding of host-virus interactions in this species.
白斑病等疾病的出现已对养殖构成威胁。尽管已有一些利用RNA测序的研究，但该物种中宿主与病毒相互作用的细胞过程大多仍不清楚。在本研究中，通过肌肉注射用对虾白斑综合征病毒（WSSV）对虾进行攻毒，虾存活了12天。利用Illumina HiSeq 2000研究了WSSV感染对虾血细胞、肝胰腺和肌肉中宿主基因表达的影响。从存活的经WSSV攻毒的虾以及正常健康虾作为对照构建的cDNA文库进行了RNA测序。两组转录组数据的比较显示，由于感染WSSV，有2644个宿主基因显著上调，2194个基因显著下调。在差异表达基因中，我们的研究在虾中发现了高迁移率族蛋白B（HMGB）、肿瘤坏死因子超家族成员（TNFSF）和c-Jun作为新的潜在候选基因，用于进一步研究以开发潜在的抗病标记。我们的研究还提供了关于存活的WSSV感染虾中基因差异表达的重要数据，这将有助于增进对该物种中宿主-病毒相互作用的理解。
