College of Textile and Garments, Southwest University, Chongqing 400715, China.
College of Textile and Garments, Southwest University, Chongqing 400715, China; Chongqing Engineering Research Center of Biomaterial Fiber and Modern Textile, Chongqing 400715, China.
Carbohydr Polym. 2017 Oct 1;173:556-565. doi: 10.1016/j.carbpol.2017.06.035. Epub 2017 Jun 9.
Spongy composites with silver nanoparticles (AgNPs) were synthesized by freeze-drying a mixture of silver nitrate (AgNO) and chitosan-l-glutamic acid (CG) derivative loaded with hyaluronic acid (HA) solution. CG/AgNP spongy composites had an interconnected porous structure and rough surfaces. When AgNPs (5-20nm) were immobilized on these spongy composites, AgNP aggregation was dependent on AgNO concentration. The spongy composites exhibited good mechanical properties, swelling, and water retention capacity. In vitro antibacterial activity showed that the CG/AgNP spongy composites effectively inhibited bacterial (Escherichia coli and Staphylococcus aureus) growth and penetration. Spongy composites containing low concentrations of AgNP were non-toxic to L929 cells, while CG/HA/AgNP spongy composites promoted wound healing, as determined by in vivo tests, wound contraction ratio, average healing time, and histological examination. These results indicate that the spongy composites can serve as effective antibacterial wound dressings.
具有银纳米粒子（AgNPs）的海绵复合材料是通过冷冻干燥载有透明质酸（HA）溶液的硝酸银（AgNO）和壳聚糖-l-谷氨酸（CG）衍生物的混合物合成的。CG/AgNP 海绵复合材料具有相互连接的多孔结构和粗糙表面。当 AgNPs（5-20nm）固定在这些海绵复合材料上时，AgNP 的聚集取决于 AgNO 浓度。海绵复合材料表现出良好的机械性能、膨胀和保水能力。体外抗菌活性表明，CG/AgNP 海绵复合材料能有效抑制细菌（大肠杆菌和金黄色葡萄球菌）的生长和渗透。含有低浓度 AgNP 的海绵复合材料对 L929 细胞无毒，而 CG/HA/AgNP 海绵复合材料通过体内试验、伤口收缩率、平均愈合时间和组织学检查促进伤口愈合。这些结果表明，海绵复合材料可用作有效的抗菌伤口敷料。
