Zhang Yahui, Zhang Xiaochen, Hu Ruiming, Yang Yang, Li Ping, Wu Qingsheng
School of Chemical Science and Engineering, School of Life Science and Technology, Shanghai Key Laboratory of Chemical Assessment and Sustainability, Tongji University Shanghai 200092 China
Taiyuan Environmental Science Research Institute Taiyuan 030002 China.
RSC Adv. 2019 Jun 6;9(31):17913-17920. doi: 10.1039/c9ra01969c. eCollection 2019 Jun 4.
Since the efficacy of antibiotics towards bacteria is decreasing over time, the rising of antibiotic emission has become an increasingly grave issue. In this study, we proposed an integrated antibacterial nanotechnology without pollution residues, which synergistically enhances the antibacterial activity of ceftazidime by using the inorganic nano-AgPO, and subsequently removes drug residues by photocatalysis. AgPO were synthesized using a simple ion-exchange method without any reducing agent or protectant. The combined antibacterial activity of AgPO and 22 kinds of antibiotics against was first studied. The results showed that AgPO and ceftazidime exhibited the best synergistic effect. Next, the synergy mechanism was proposed, the non-chemical bond forces between AgPO and ceftazidime was determined by zeta potential analyzer, X-ray photoelectron spectroscopy (XPS) and infrared spectroscopy (IR). The interaction between antimicrobials and bacteria was further demonstrated by surface plasma resonance spectroscopy (SPR), scanning electron microscopy (SEM) and propidium iodide (PI) staining. In addition, the production of reactive oxygen species (ROS), the induction of oxidative stress and dissolution of silver ions in AgPO were studied and found out that only under light, could the ROS be generated. In conclusion, the synergistic effect of AgPO and ceftazidime is responsible for the joint destruction of cell wall.
由于抗生素对细菌的疗效会随着时间推移而降低，抗生素排放的增加已成为一个日益严峻的问题。在本研究中，我们提出了一种无污染物残留的综合抗菌纳米技术，该技术通过使用无机纳米AgPO协同增强头孢他啶的抗菌活性，随后通过光催化去除药物残留。AgPO采用简单的离子交换法合成，无需任何还原剂或保护剂。首先研究了AgPO与22种抗生素对[具体对象未提及]的联合抗菌活性。结果表明，AgPO与头孢他啶表现出最佳的协同效应。接下来，提出了协同机制，通过zeta电位分析仪、X射线光电子能谱（XPS）和红外光谱（IR）确定了AgPO与头孢他啶之间的非化学键力。通过表面等离子体共振光谱（SPR）、扫描电子显微镜（SEM）和碘化丙啶（PI）染色进一步证明了抗菌剂与细菌之间的相互作用。此外，研究了活性氧（ROS）的产生、氧化应激的诱导以及AgPO中银离子的溶解，发现只有在光照下才能产生ROS。总之，AgPO与头孢他啶的协同作用导致细胞壁的联合破坏。
