Instituto de Ciencia y Tecnología Dr César Milstein, Fundación Pablo Cassará, CONICET, Saladillo 2468, C1440FFX Ciudad de Buenos Aires, Argentina.
Embrapa Recursos Genéticos e Biotecnología and Centro APTA Citros Sylvio Moreira, Instituto Agronômico de Campinas, Cordeiropolis, Sao Pablo, Brazil.
Microbiology (Reading). 2013 Sep;159(Pt 9):1911-1919. doi: 10.1099/mic.0.064709-0. Epub 2013 Jun 27.
Xanthomonas citri subsp. citri (Xcc) develops a biofilm structure both in vitro and in vivo. Despite all the progress achieved by studies regarding biofilm formation, many of its mechanisms remain poorly understood. This work focuses on the identification of new genes involved in biofilm formation and how they are related to motility, virulence and chemotaxis in Xcc. A Tn5 library of approximately 6000 Xcc (strain 306) mutants was generated and screened to search for biofilm formation defective strains. We identified 23 genes not previously associated with biofilm formation. The analysis of the 23 mutants not only revealed the involvement of new genes in biofilm formation, but also reinforced the importance of exopolysaccharide production, motility and cell surface structures in this process. This collection of biofilm-defective mutants underscores the multifactorial genetic programme underlying the establishment of biofilm in Xcc.
柑橘木虱黄单胞菌亚种柑橘（Xcc）在体外和体内都能形成生物膜结构。尽管关于生物膜形成的研究已经取得了所有的进展，但许多机制仍未被充分理解。本工作专注于鉴定参与生物膜形成的新基因，以及它们如何与柑橘木虱黄单胞菌亚种的运动性、毒性和趋化性相关。大约 6000 个柑橘木虱黄单胞菌（菌株 306）突变体的 Tn5 文库被生成并筛选，以寻找生物膜形成缺陷型菌株。我们鉴定了 23 个以前与生物膜形成无关的基因。对 23 个突变体的分析不仅揭示了新基因在生物膜形成中的参与，还加强了胞外多糖产生、运动性和细胞表面结构在这一过程中的重要性。这组生物膜缺陷型突变体强调了柑橘木虱黄单胞菌亚种生物膜形成的多因素遗传程序。
