Rogers J, Mufson E J
L. J. Roberts Center, Institute for Biogerontology Research, Sun City, AZ 85351.
Neurobiol Aging. 1990 Jul-Aug;11(4):477-9. doi: 10.1016/0197-4580(90)90016-s.
Immune system associated markers have recently been reported in Alzheimer's disease (AD) brain. However, previous studies have not consistently revealed these antigens in the central nervous system. Immunohistochemistry of HLA-DR and other immune antigens (e.g., CD4+, IL-2R) in AD cortical tissue was compared using paraformaldehyde-fixed versus snap-frozen unfixed tissue. In addition, a free-floating immunohistochemistry method was contrasted with an on slide procedure. Even with an HLA-DR antibody known to work well on fixed tissue, unfixed tissue gave superior results. Free-floating immunohistochemistry also increased sensitivity. Where morphologic considerations are paramount, brief fixation with free-floating methods provides a reasonable alternative.
免疫系统相关标志物最近在阿尔茨海默病（AD）大脑中被报道。然而，先前的研究并未始终在中枢神经系统中发现这些抗原。使用多聚甲醛固定组织与速冻未固定组织对比了AD皮质组织中HLA-DR和其他免疫抗原（如CD4+、IL-2R）的免疫组织化学。此外，还对比了游离切片免疫组织化学方法与载玻片上操作方法。即便使用一种已知在固定组织上效果良好的HLA-DR抗体，未固定组织仍给出了更好的结果。游离切片免疫组织化学也提高了敏感性。在形态学考量至关重要的情况下，采用游离切片方法进行短暂固定提供了一种合理的替代方案。
