核苷构象。19. 温度和pH值对鸟苷磷酸盐构象的影响。
Necleoside conformations. 19. Temperature and pH effects on the conformation of guanosine phosphates.
作者信息
Son T D, Guschlbauer W
出版信息
Nucleic Acids Res. 1975 Jun;2(6):873-86. doi: 10.1093/nar/2.6.873.
Abstract
Proton magnetic resonance spectra at 250 MHz were measured as a function of temperature and pH of the three guanosine phosphates. From these data and previously published work the conformational parameters of these compounds were determinated. The phosphate group of Guo-5'-P changes its conformation around the C-O bond and its rotation is relatively slow at 20 degrees. At neutral pD the S conformation is favoured and the N form at acid pD. This conformational change is paralleled by a change in exocyclic rotamer distribution and takes place at the pK of the protonation of the base on N-7. Although correlation appears to exist between the various conformations, notable exceptions exist.
摘要
在250兆赫下测量了三种鸟苷磷酸盐的质子磁共振谱，该谱是温度和pH值的函数。根据这些数据以及先前发表的研究成果，确定了这些化合物的构象参数。Guo-5'-P的磷酸基团围绕C-O键改变其构象，并且在20摄氏度时其旋转相对较慢。在中性pD时，S构象占优势，而在酸性pD时为N构象。这种构象变化与环外旋转异构体分布的变化平行，并且发生在N-7位碱基质子化的pK值处。尽管各种构象之间似乎存在相关性，但也存在明显的例外情况。
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