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苦参和窃衣提取物高效液相色谱馏分的气相色谱/质谱分析及其对犬新孢子虫的体外抗新孢子虫作用。

GC/MS analysis of high-performance liquid chromatography fractions from Sophora flavescens and Torilis japonica extracts and their in vitro anti-neosporal effects on Neospora caninum.

作者信息

Seo Hun-Su, Kim Kyoung Hee, Kim Dae-Yong, Park Bong-Kyun, Shin Nam-Shik, Kim Jae-Hoon, Youn Heejeong

机构信息

Pfizer Animal Health, Seoul 135-784, Korea.

出版信息

J Vet Sci. 2013;14(3):241-8. doi: 10.4142/jvs.2013.14.3.241. Epub 2013 Jun 30.

DOI:10.4142/jvs.2013.14.3.241
PMID:23820198
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3788148/
Abstract

We analyzed alcoholic extracts of herbs possessing anti-neosporal activity against Neospora (N.) caninum. To identify the chemical components of Sophora (S.) flavescens and Torilis (T.) japonica associated with anti-neosporal activity, specific fractions were isolated by high-performance liquid chromatography (HPLC). In vitro activity of the fractions against N. caninum was then assessed. Gas chromatography/ mass spectrometry (GC/MS) was used to identify and quantify specific anti-neosporal molecules in the herbal extracts. Almost all HPLC fractions of S. flavescens and T. japonica had higher levels of anti-neosporal activity compared to the not treated control. Active constituents of the extracts were sophoridane, furosardonin A, and tetraisopropylidene-cyclobutane in S. flavescens; 5,17-β-dihydroxy-de-A-estra-5,7,9,14-tetraene, furanodiene, and 9,12-octadecadienoic acid (Z,Z)-(CAS,1) in T. japonica.

摘要

我们分析了对犬新孢子虫具有抗新孢子虫活性的草药乙醇提取物。为了鉴定与抗新孢子虫活性相关的苦参和日本窃衣的化学成分，通过高效液相色谱（HPLC）分离了特定馏分。然后评估这些馏分对犬新孢子虫的体外活性。气相色谱/质谱联用（GC/MS）用于鉴定和定量草药提取物中的特定抗新孢子虫分子。与未处理的对照相比，苦参和日本窃衣的几乎所有HPLC馏分都具有更高水平的抗新孢子虫活性。提取物的活性成分在苦参中为槐烷、呋甾皂苷元A和四异丙基环丁烷；在日本窃衣中为5,17-β-二羟基-去-A-雌甾-5,7,9,14-四烯、呋喃二烯和9,12-十八碳二烯酸（Z,Z）-（CAS,1）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1f/3788148/5ae793661668/jvs-14-241-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1f/3788148/20a8244f75a3/jvs-14-241-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1f/3788148/a2da7d558592/jvs-14-241-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1f/3788148/cb00a8c3f5b2/jvs-14-241-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1f/3788148/654061b4389a/jvs-14-241-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1f/3788148/9294db7f01f2/jvs-14-241-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1f/3788148/5ae793661668/jvs-14-241-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1f/3788148/20a8244f75a3/jvs-14-241-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1f/3788148/a2da7d558592/jvs-14-241-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1f/3788148/cb00a8c3f5b2/jvs-14-241-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1f/3788148/654061b4389a/jvs-14-241-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1f/3788148/9294db7f01f2/jvs-14-241-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef1f/3788148/5ae793661668/jvs-14-241-g006.jpg

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