Ye Guan, Zhu Hai-Yan, Li Zhi-Xiong, Ma Chun-Hui, Fan Ming-Song, Sun Zhao-Lin, Huang Cheng-Gang
Shanghai Institute of Materia Medica, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
Biomed Chromatogr. 2007 Jun;21(6):655-60. doi: 10.1002/bmc.805.
Anti-DHBV (duck hepatitis B virus) activity was found in the aqueous extracts of Sophora flavescens Ait. in vivo. Liquid chromatography/electrospray ionization ion trap mass spectrometry was applied to characterize the components in duck serum after oral administration of S. flavescens extract. Oxymatrine (1), sophoranol (2), sophoridine (3) and matrine (4) were identified in the serum. Further research on the four compounds was evaluated for their antiviral activity against HBV (hepatitis B virus) in cell culture. The results suggested that oxymatrine, sophoranol and matrine were the efficacy substances for anti-HBV activity in aqueous extracts of S. flavescens Ait.
在体内研究中发现苦参水提取物具有抗鸭乙型肝炎病毒（DHBV）活性。采用液相色谱/电喷雾电离离子阱质谱法对口服苦参提取物后鸭血清中的成分进行表征。在血清中鉴定出氧化苦参碱（1）、槐醇（2）、槐定碱（3）和苦参碱（4）。进一步研究了这四种化合物在细胞培养中对乙型肝炎病毒（HBV）的抗病毒活性。结果表明，氧化苦参碱、槐醇和苦参碱是苦参水提取物中抗HBV活性的有效物质。
