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敲低 BMI1 表达抑制人膀胱癌 T24 细胞的增殖和侵袭。

Knockdown BMI1 expression inhibits proliferation and invasion in human bladder cancer T24 cells.

机构信息

Department of Urology, The Second Affiliated Hospital of Sun Yat-sen University, No. 107 Yan-jiang West Road, Guangzhou, Guangdong Province, 510120, People's Republic of China,

出版信息

Mol Cell Biochem. 2013 Oct;382(1-2):283-91. doi: 10.1007/s11010-013-1745-0. Epub 2013 Jul 3.

DOI:10.1007/s11010-013-1745-0
PMID:23820733
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3771375/
Abstract

B cell-specific moloney murine leukemia virus integration site 1 (BMI1) is a transcriptional repressor of polycomb repressive complex 1, which is involved in the proliferation, senescence, migration, and tumorigenesis of cancer. Experimental researchers have convincingly linked BMI1 to tumorigenesis. However, there is no study about the issue on the role of BMI1 in the proliferation, apoptosis, and migration of bladder cancer. To address this question, we examined the expression of BMI1 in bladder cancer tissues and used siRNA to knockdown BMI1 expression in bladder cancer T24 cells. Then we tested the cell proliferation by CCK8 assay and soft agar colony formation assay, apoptosis by flow cytometry assay, and cell invasiveness by transwell migration assay. Our results revealed that BMI1 promoted proliferation, migration, invasion, and progression in bladder cancer. Over-expression of BMI1 was correlated with tumor clinic-pathological features. BMI1 siRNA effectively inhibited bladder cancer cell proliferation and migration in vitro, and it promoted bladder cancer invasion, maybe by causing epithelial-to-mesenchymal transition. Our findings suggested that BMI1 may represent a novel diagnostic marker and a therapeutic target for bladder cancer, and deserves further investigation.

摘要

B 细胞特异性莫洛尼鼠白血病病毒整合位点 1（BMI1）是多梳抑制复合物 1 的转录抑制剂，参与癌症的增殖、衰老、迁移和致瘤性。实验研究人员令人信服地将 BMI1 与肿瘤发生联系起来。然而，目前还没有关于 BMI1 在膀胱癌增殖、凋亡和迁移中的作用的研究。为了解决这个问题，我们检测了膀胱癌组织中 BMI1 的表达，并使用 siRNA 敲低膀胱癌 T24 细胞中的 BMI1 表达。然后，我们通过 CCK8 检测、软琼脂克隆形成检测、流式细胞术检测、Transwell 迁移检测来检测细胞增殖、凋亡和迁移。我们的结果表明，BMI1 促进了膀胱癌的增殖、迁移、侵袭和进展。BMI1 的过表达与肿瘤临床病理特征相关。BMI1 siRNA 有效抑制了膀胱癌细胞在体外的增殖和迁移，并促进了膀胱癌的侵袭，可能是通过引起上皮间质转化。我们的研究结果表明，BMI1 可能成为膀胱癌的一种新的诊断标志物和治疗靶点，值得进一步研究。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21e5/3771375/95907f031bc1/11010_2013_1745_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21e5/3771375/59179ad34e07/11010_2013_1745_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21e5/3771375/b2f8002809fd/11010_2013_1745_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21e5/3771375/a64e0ef4eaa1/11010_2013_1745_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21e5/3771375/ab8720f3afb9/11010_2013_1745_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21e5/3771375/cc74135f125a/11010_2013_1745_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21e5/3771375/a01d6c19109e/11010_2013_1745_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21e5/3771375/1be865e6dcc6/11010_2013_1745_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21e5/3771375/8788bc6184a5/11010_2013_1745_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21e5/3771375/95907f031bc1/11010_2013_1745_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21e5/3771375/59179ad34e07/11010_2013_1745_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21e5/3771375/b2f8002809fd/11010_2013_1745_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21e5/3771375/a64e0ef4eaa1/11010_2013_1745_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21e5/3771375/ab8720f3afb9/11010_2013_1745_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21e5/3771375/cc74135f125a/11010_2013_1745_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21e5/3771375/a01d6c19109e/11010_2013_1745_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21e5/3771375/1be865e6dcc6/11010_2013_1745_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21e5/3771375/8788bc6184a5/11010_2013_1745_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21e5/3771375/95907f031bc1/11010_2013_1745_Fig9_HTML.jpg

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