Department of Cardiothoracic Surgery, Shanghai Children's Medical Center, Shanghai Jiao Tong University School of Medicine, 1678 Dong Fang Road, Shanghai 200127, China.
Regen Med. 2013 Jul;8(4):425-36. doi: 10.2217/rme.13.29.
To study the feasibility of electrospun collagen-poly(L-lactic acid-co-ε-caprolactone) (collagen-PLCL) membranes for cartilage tissue engineering.
MATERIALS & METHODS: Characteristics and mechanical properties of collagen-PLCL membranes were analyzed. The cell affinity of collagen-PLCL membranes with chondrocytes was also assessed. Then, the cell-scaffold constructs were engineered with collagen-PLCL membranes seeded chondrocytes by a sandwich model. After culture for 1 week in vitro, the constructs were implanted subcutaneously into nude mice for 4, 8 and 12 weeks, followed by evaluation of the quality of neocartilage.
Collagen-PLCL membranes exhibited excellent balanced properties without cytotoxicity. With the extension of implantation time in vivo, the constructs revealed more cartilage-like tissue especially at 8 and 12 weeks. The Young's modulus of the constructs also significantly increased and neared that of native cartilage at 12 weeks postimplantation.
We suggest that collagen-PLCL membranes facilitate the formation of cartilage and thus may represent a promising scaffold for cartilage tissue engineering.
研究静电纺胶原-聚（L-丙交酯-co-ε-己内酯）（胶原-PLCL）膜在软骨组织工程中的可行性。
分析胶原-PLCL 膜的特性和力学性能。评估胶原-PLCL 膜与软骨细胞的细胞亲和性。然后，通过三明治模型将胶原-PLCL 膜接种软骨细胞，构建细胞-支架复合物。体外培养 1 周后，将构建物皮下植入裸鼠体内 4、8 和 12 周，然后评估新生软骨的质量。
胶原-PLCL 膜表现出优异的平衡性能，无细胞毒性。随着体内植入时间的延长，构建物显示出更多的软骨样组织，特别是在 8 周和 12 周时。构建物的杨氏模量也显著增加，并在植入后 12 周接近天然软骨。
我们认为胶原-PLCL 膜有助于软骨的形成，因此可能代表软骨组织工程有前途的支架。
