多表位融合蛋白Eg mefAg-1作为绵羊囊型棘球蚴病血清学诊断候选抗原

Multi-Epitope Fusion Protein Eg mefAg-1 as a Serodiagnostic Candidate for Cystic Echinococcosis in Sheep.

作者信息

Tianli Liu, Xifeng Wang, Zhenzhong Tian, Lixia Wang, Xingxing Zhang, Jun Qiao, Qingling Meng, Shasha Gong, Ying Chen, Xuepeng Cai

机构信息

College of Animal Science & Technology, Shihezi University, Shihezi, Xinjiang 832003, China.

State Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Science, Shihezi, Xinjiang 832000, China.

出版信息

Korean J Parasitol. 2019 Feb;57(1):61-67. doi: 10.3347/kjp.2019.57.1.61. Epub 2019 Feb 26.

DOI:10.3347/kjp.2019.57.1.61

PMID:30840802

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6409221/

Abstract

Cystic echinococcosis (CE) in sheep is a hazardous zoonotic parasitic disease that is caused by Echinococcus granulosus (Eg). At present, serological test is an important diagnostic method for Eg infection in domestic animals. Here, a fusion protein Eg mefAg-1 harboring 8 dominant B-cell epitopes of Eg such as antigen B, tetraspanin 1, tetraspanin 6, reticulon and Eg95 was produced in E. coli and evaluated for CE in sheep by indirect ELISA. Eg mefAg-1 showed in ELISA a high sensitivity (93.41%) and specificity (99.31%), with a coincidence rate of 97.02%. Overall, it is suggested that the Eg mefAg-1 could be a potential antigen candidate for CE serodiagnosis in sheep.

摘要

绵羊囊型包虫病（CE）是由细粒棘球绦虫（Eg）引起的一种危险的人畜共患寄生虫病。目前，血清学检测是家畜Eg感染的重要诊断方法。在此，一种包含Eg的8个显性B细胞表位（如抗原B、四跨膜蛋白1、四跨膜蛋白6、网质蛋白和Eg95）的融合蛋白Eg mefAg-1在大肠杆菌中产生，并通过间接ELISA对绵羊CE进行评估。Eg mefAg-1在ELISA中显示出高敏感性（93.41%）和特异性（99.31%），符合率为97.02%。总体而言，提示Eg mefAg-1可能是绵羊CE血清学诊断的潜在抗原候选物。