共振光散射技术作为一种新工具，用于确定抗癌药物冬凌草素与 DNA 的结合模式。
Resonance light scattering technique as a new tool to determine the binding mode of anticancer drug oridonin to DNA.
机构信息
Department of Chemistry, Shantou University, Shantou 515063, China.
出版信息
Eur J Med Chem. 2013 Aug;66:380-7. doi: 10.1016/j.ejmech.2013.05.042. Epub 2013 Jun 14.
In this study, a sensitive and reliable assay has been developed to determine the binding mode of oridonin to DNA in vitro using resonance light scattering (RLS) technique. The binding mode of ORI with DNA has been confirmed by measuring the changes of RLS intensity under different pH and ionic strength. It is the ORI that is intercalated into the double helix DNA to destroy its template function and inhibit the synthesis of DNA. Moreover, the RLS assay result was validated by seasoned vitro methods. The proposed RLS assay is not only an unprecedented one for primarily determine the binding mode of oridonin to DNA, but also a simple, sensitive, objective and straightforward method for the detection of ORI.
在这项研究中，我们开发了一种灵敏可靠的测定方法，即利用共振光散射（RLS）技术来确定冬凌草甲素与 DNA 的体外结合模式。通过测量不同 pH 值和离子强度下 RLS 强度的变化，证实了 ORI 与 DNA 的结合模式。ORI 通过嵌入 DNA 双螺旋结构来破坏其模板功能并抑制 DNA 的合成。此外，RLS 检测结果还通过传统的体外方法进行了验证。该 RL S 检测方法不仅为确定冬凌草甲素与 DNA 的结合模式提供了一种前所未有的方法，而且为检测冬凌草甲素提供了一种简单、灵敏、客观和直接的方法。
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