维多利亚蓝B与脱氧核糖核酸相互作用的共振光散射研究及其在DNA测定中的应用

[Resonance light scattering study on the interaction of Victory blue-B with deoxyribonucleic acid and application to DNA assay].

作者信息

Su Jie-shu, Chen Xiao-ming, Long Yun-fei, Li Wei, Luo Hai-tang

机构信息

Institute of Chemistry, Xiangtan University, Xiangtan 411105, China.

出版信息

Guang Pu Xue Yu Guang Pu Fen Xi. 2004 Jan;24(1):37-40.

PMID:15768971

Abstract

The enhancement of the Resonance Light Scattering (RLS) due to Victory blue-B on the molecular surface of DNA in alkalescence solution was studied. A method of resonance light scattering was developed to determine DNA. Deoxyribonucleic acid (DNA) was studied in the range of pH 9.0-10.5. The effects of interference substances and optimization of procedure were investigated and a new method was established for the determination of DNA at nanogram level based on RLS measurement. The linear range of the DNA is 0-3.0 mg x L(-1) with a correlation coefficient of 0.9978. The detection limit is 21.5 microg x L(-1). The method has been applied to the determination of DNA in mixed samples with satisfactory result.

摘要

研究了在碱性溶液中维多利亚蓝B对DNA分子表面共振光散射（RLS）的增强作用。建立了一种用于测定DNA的共振光散射方法。在pH 9.0 - 10.5范围内对脱氧核糖核酸（DNA）进行了研究。考察了干扰物质的影响并对实验程序进行了优化，基于RLS测量建立了一种用于测定纳克级DNA的新方法。DNA的线性范围为0 - 3.0 mg·L⁻¹，相关系数为0.9978。检测限为21.5 μg·L⁻¹。该方法已应用于混合样品中DNA的测定，结果令人满意。