Becker Hilton, Vazquez Oscar Adrian, Rosen Tans
Charles E. Schmidt College of Medicine, Florida Atlantic University, Boca Raton, Fla.
Clinic of Plastic Surgery, Boca Raton, Fla.
Plast Reconstr Surg Glob Open. 2021 Mar 23;9(3):e3471. doi: 10.1097/GOX.0000000000003471. eCollection 2021 Mar.
Fat is an active and dynamic tissue composed of adipocytes supported by a structural framework known as the stromal vascular fraction (SVF). SVF is traditionally isolated by enzymatic processing, but new methods are being investigated to isolate it mechanically. Recent studies propose that fat harvested with larger cannulas has a higher survival rate, most likely due to a higher concentration of SVF.
Lipoaspirates were obtained from 10 patients who underwent elective liposuction using a 5-mm and a 1-mm cannula attached to a syringe using standard pressure. The fat was aspirated from the same area at adjacent sites. An estimated 5-mm fat particles were also cut down to 1-mm using a micronizer (Marina Medical). A 5-cm volume of each sample was compressed through a 0.5-mm opening strainer and rinsed with normal saline to extrude the oil. The resultant SVF left on the strainer was then measured in a 1-cm syringe.
The volume extracted from a 5-mm cannula (mean, 0.23 cm; SD, 0.10) versus a 1-mm cannula (mean, 0.11 cm; SD, 0.06) was statistically significant ( = 0.009). An H&E-stained slide from the SVF was obtained for confirmation. Finally, 5-mm fat particles cut down to 1-mm particles using the micronizer resulted in an average volume of 0.20 cm, which was higher than the average volume harvested with a 1-mm cannula.
Harvesting with a 5-mm cannula resulted in significantly more SVF than harvesting with a 1-mm cannula. Resizing fat particles harvested with a larger cannula down to 1-mm resulted in higher SVF than SVF obtained with a 1-mm cannula directly.
脂肪是一种活跃的动态组织，由脂肪细胞组成，由称为基质血管成分（SVF）的结构框架支撑。传统上，SVF是通过酶处理分离的，但目前正在研究机械分离的新方法。最近的研究表明，使用较大套管采集的脂肪具有更高的存活率，这很可能是由于SVF浓度较高。
从10例接受择期吸脂术的患者身上获取脂肪抽吸物，使用连接到注射器的5毫米和1毫米套管，采用标准压力。脂肪从相邻部位的同一区域吸出。使用微粉化器（Marina Medical）将估计5毫米的脂肪颗粒也切割成1毫米。将每个样本5厘米的体积通过0.5毫米开口的滤网压缩，并用生理盐水冲洗以挤出油脂。然后在1厘米的注射器中测量留在滤网上的所得SVF。
从5毫米套管中提取的体积（平均值为0.23厘米；标准差为0.10）与1毫米套管中提取的体积（平均值为0.11厘米；标准差为0.06）在统计学上具有显著差异（P = 0.009）。获取了一张来自SVF的苏木精-伊红染色切片用于确认。最后，使用微粉化器将5毫米的脂肪颗粒切割成1毫米的颗粒，平均体积为0.20厘米，高于用1毫米套管采集的平均体积。
使用5毫米套管采集的SVF明显多于使用1毫米套管采集的。将用较大套管采集的脂肪颗粒尺寸调整为1毫米后，得到的SVF高于直接用1毫米套管获得的SVF。
